From several gnathostome species the complete internal transcribed spacer ITS-2 ribosomal DNA (rDNA) repeat sequence and a fragment of the 5.8S rDNA were obtained by direct polymerase chain reaction cycle-sequencing and silver-staining methods. The size of the complete ITS-1 sequence in agarose gel electrophoresis was also obtained. The ITS-2 enabled the differentiation of Gnathostoma spinigerum from Thailand and Gnathostoma binucleatum from Mexico and Ecuador and confirmed the validity of the latter. Gnathostoma turgidum, Gnathostoma sp. I (=Gnathostoma procyonis sensu Almeyda-Artigas et al., 1994), and Gnathostoma sp. II (=G. turgidum sensu Foster, 1939 pro parte), all from Mexico, proved to be independent species, but Gnathostoma sp. III, also from Mexico, could not be differentiated from G. turgidum. In Mexico and Ecuador, gnathostomes involved in human infection and that had been classified as G. spinigerum belong to G. binucleatum. The 5.8S rDNA sequences of the 6 Gnathostoma species studied were identical. The results of the ITS-1 agreed with those results of ITS-2.
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June 2000
SYSTEMATICS-PHYLOGENETICS|
June 01 2000
ITS-2 rDNA SEQUENCING OF GNATHOSTOMA SPECIES (NEMATODA) AND ELUCIDATION OF THE SPECIES CAUSING HUMAN GNATHOSTOMIASIS IN THE AMERICAS
Roberto J. Almeyda-Artigas;
Roberto J. Almeyda-Artigas
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
*Present address: Laboratorio de Sanidad Acuícola y Parasitología Molecular, Departamento de “El Hombre y su Ambiente,” Universidad Autónoma Metropolitana–Xochimilco, Calzada del Hueso No. 1100, Colonia Villa Quietud, Delegación Coyoacán, 04960, México D.F., México.
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M. Dolores Bargues;
M. Dolores Bargues
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
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Santiago Mas-Coma
Santiago Mas-Coma
Departamento de Parasitología, Facultad de Farmacia, Universidad de Valencia, Av. Vicente Andrés Estellés s/n, 46100 Burjassot, Valencia, Spain
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J Parasitol (2000) 86 (3): 537–544.
Citation
Roberto J. Almeyda-Artigas, M. Dolores Bargues, Santiago Mas-Coma; ITS-2 rDNA SEQUENCING OF GNATHOSTOMA SPECIES (NEMATODA) AND ELUCIDATION OF THE SPECIES CAUSING HUMAN GNATHOSTOMIASIS IN THE AMERICAS. J Parasitol 1 June 2000; 86 (3): 537–544. doi: https://doi.org/10.1645/0022-3395(2000)086[0537:IRSOGS]2.0.CO;2
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