Serum samples of 31 amebic liver abscess (ALA) patients, 8 amebic hepatitis (AH) patients, and 60 controls were tested for anti-amebic IgG by enzyme-linked immunosorbent assay (ELISA) and indirect fluorescent antibody tests (IFAT). Sera of 29 (93.6%) ALA and 6 (75%) AH patients and 2 (3.3%) control subjects were positive by IFAT. Anti-amebic antibody titer above the cutoff point (= 0.168; x̄ + 2 SD of control sera) was observed in sera of 27 (87%) ALA, 4 (50%) AH, and 1 (1.7%) control by ELISA. All the 8 pus samples were positive for anti-amebic antibodies by IFAT and ELISA. Sensitivity of ELISA was 87% for ALA, with a positive predictive value of 0.96, and 50% for AH cases, with a positive predictive value of 0.80. The sensitivity of IFAT was 93.6% for ALA, with a positive predictive value of 0.94, and 75% for AH, with a positive predictive value of 0.75. When pus samples were tested, the sensitivity was 100% for both tests. The specificity was 98.3% for ELISA and 96.7% for IFAT. Although not significant, IFAT was found more sensitive than ELISA (P > 0.05).
EVALUATION OF INDIRECT FLUORESCENT ANTIBODY TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DIAGNOSIS OF HEPATIC AMEBIASIS IN BANGLADESH
- Views Icon Views
- Share Icon Share
- Search Site
S. M. Shamsuzzaman, Rashidul Haque, S. K. Ruhul Hasin, Yoshihisa Hashiguchi; EVALUATION OF INDIRECT FLUORESCENT ANTIBODY TEST AND ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DIAGNOSIS OF HEPATIC AMEBIASIS IN BANGLADESH. J Parasitol 1 June 2000; 86 (3): 611–615. doi: https://doi.org/10.1645/0022-3395(2000)086[0611:EOIFAT]2.0.CO;2
Download citation file: