Negatively charged liposomes, proposed as potential vaccine adjuvants, have been extensively studied in association with various antigens. In the present study, we investigated the adjuvanicity of negatively charged liposomes to enhance the protective immunity of membrane antigens of Leishmania donovani promastigotes (LAg). In comparison to the control mice immunized with phosphate-buffered saline and empty liposomes, immunization with free LAg led to significant levels of protection against infection with virulent promastigotes. Encapsulation of LAg in liposomes also induced effective protection. However, the level of protection by LAg–liposome was not significantly different from that induced by free LAg. Investigation of the immune responses showed, in contrast to free LAg, that immunization with LAg–liposome elicited strong antibody responses. IgG isotype analysis revealed the presence of all 4 isotypes. However, the titer of IgG1 was significantly higher than IgG2a, IgG2b, and IgG3. Following infection, stimulation of IgG and IgG isotypes did not differ in the different immunization groups. Delayed-type hypersensitivity (DTH) analysis after immunization showed significant induction by LAg and LAg–liposomes, in comparison to controls. With infection, again, the level of DTH in all the groups became almost comparable. Stimulation of insufficient cellular response, as reflected by DTH and potentiation of IgG1 over IgG2a, IgG2b, and IgG3 suggest a dominance of Th2 response with this liposome–antigen formulation, resulting in weak protection against visceral leishmaniasis.
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August 2000
IMMUNOLOGY|
August 01 2000
INDUCTION OF PARTIAL PROTECTION AGAINST LEISHMANIA DONOVANI BY PROMASTIGOTE ANTIGENS IN NEGATIVELY CHARGED LIPOSOMES Available to Purchase
Farhat Afrin;
Farhat Afrin
Leishmania Group, Indian Institute of Chemical Biology, Calcutta 700032, India
*Present address. Center for Biotechnology Jamia Hamdard, Hamdard University, New Delhi 110062, India.
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Khairul Anam;
Khairul Anam
Leishmania Group, Indian Institute of Chemical Biology, Calcutta 700032, India
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Nahid Ali
Nahid Ali
Leishmania Group, Indian Institute of Chemical Biology, Calcutta 700032, India
†Corresponding author at: Indian Institute of Chemical Biology 4, Raja S.C. Mullick Road, Calcutta 700032, India.
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J Parasitol (2000) 86 (4): 730–735.
Citation
Farhat Afrin, Khairul Anam, Nahid Ali; INDUCTION OF PARTIAL PROTECTION AGAINST LEISHMANIA DONOVANI BY PROMASTIGOTE ANTIGENS IN NEGATIVELY CHARGED LIPOSOMES. J Parasitol 1 August 2000; 86 (4): 730–735. doi: https://doi.org/10.1645/0022-3395(2000)086[0730:IOPPAL]2.0.CO;2
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