The presence of Toxoplasma gondii DNA was detected in sera of acutely infected mice by polymerase chain reaction. Adult mice were inoculated intraperitoneally with 5 × 103 T. gondii RH strain tachyzoites. Five mice were killed every 3 hr from 3 to 21 hr post infection (PI) and every day from 1 to 7 days PI. Toxoplasma gondii DNA was first detected in 60% of the infected mice 18 hr PI and in 100% of the animals 21 hr PI and from 1 to 7 days PI. No mice survived longer than 7 days.
Detection of Toxoplasma gondii by Polymerase Chain Reaction in Sera of Acutely Infected Mice
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J. Hafid, D. Guichard, P. Flori, T. Bourlet, H. Raberin, C. Genin, R. Tran Manh Sung; Detection of Toxoplasma gondii by Polymerase Chain Reaction in Sera of Acutely Infected Mice. J Parasitol 1 August 2000; 86 (4): 857–859. doi: https://doi.org/10.1645/0022-3395(2000)086[0857:DOTGBP]2.0.CO;2
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