Schistosoma mansoni egg antigens are mostly responsible for the granulomatous pathology in human intestinal schistosomiasis. Several previous studies have indicated that the induction of an immune response against some parasite enzymes may protect against pathology. The present work was designed to identify enzyme activities present in a standard soluble egg antigen (SEA) preparation. Simple colorimetric analyses were performed incubating SEA with 2-naphthyl, 2-naphthylamide (2NA), or p-nitrophenyl substrates at different pHs in the absence of added effectors. Results showed prominent acid phosphatase (pH 5.4), alkaline phosphatase (pH 8.5), and N-acetyl-β-glucosaminidase (pH 5.4) activities. Relevant peptidase activities were also detected at pH 6.5–7.5 against 2NA derivatives of (1) aliphatic (α-Ala > β-Ala > Leu > Met > S-benzyl-Cys), polar (Ser > Gln), basic (Arg > Lys > ornithine), and acidic (Glu) amino acids; (2) dipeptides: X-Ala (X = Gly > Leu > Lys > Asp), X-Arg (X = Ala > Arg > Phe > Gly > Pro > Asp), Ser-Met, and Phe-Pro; and (3) tripeptides (Ala-Phe-Pro > Phe-Pro-Ala). The data demonstrated that S. mansoni SEA contains a rich set of hydrolases with different specificities that might play a role in the egg physiology and possibly also in the host–parasite relationships.
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October 2000
Research Article|
October 01 2000
Enzyme Activities in Schistosoma mansoni Soluble Egg Antigen
I. M. Cesari;
I. M. Cesari
aLaboratorio de Inmunoparasitología, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (I.V.I.C.), Apartado 21827, Caracas 1020-A, Venezuela
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D. E. Ballen;
D. E. Ballen
aLaboratorio de Inmunoparasitología, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (I.V.I.C.), Apartado 21827, Caracas 1020-A, Venezuela
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T. Perrone;
T. Perrone
aLaboratorio de Inmunoparasitología, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas (I.V.I.C.), Apartado 21827, Caracas 1020-A, Venezuela
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O. Oriol;
O. Oriol
bUPR9021, Immunochimie des Peptides et des Virus, I.B.M.C., 15 rue Descartes, F-67084, Strasbourg, France
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J. Hoebeke;
J. Hoebeke
bUPR9021, Immunochimie des Peptides et des Virus, I.B.M.C., 15 rue Descartes, F-67084, Strasbourg, France
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D. Bout
D. Bout
cImmunologie des Maladies Infectieuses, 31 Av. Monge, U.F.R. des Sciences Pharmaceutiques, Université de Tours, France
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J Parasitol (2000) 86 (5): 1137–1140.
Citation
I. M. Cesari, D. E. Ballen, T. Perrone, O. Oriol, J. Hoebeke, D. Bout; Enzyme Activities in Schistosoma mansoni Soluble Egg Antigen. J Parasitol 1 October 2000; 86 (5): 1137–1140. doi: https://doi.org/10.1645/0022-3395(2000)086[1137:EAISMS]2.0.CO;2
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