The Schistosoma mansoni soluble adult worm antigen (SAWA) bands of 62/60 kDa were found to contain immunodominant T-cell immunogen(s) in irradiated cercariae-immunized Swiss and C57BL/6 mice. In the present study, spleen T cells of BALB/c mice immunized twice with ultraviolet light–irradiated cercariae proliferated and produced interleukin 4 in response to the 62/60-kDa SAWA bands in T-cell western assays. To characterize the 62/60-kDa bands, an adult S. mansoni worm cDNA expression library constructed in λgt11 was immunoscreened with serum of mice immunized with the 62/60-kDa antigens, and the immunoreactive cDNA inserts were sequenced. Purified 62- and 60-kDa proteins were used for amino acid microsequencing and for immunization studies in Swiss, C57BL/6, and BALB/c mice and rabbits. Taken together, the data indicated that the 60-kDa molecules are poorly immunogenic in mice and rabbits, whereas the 62-kDa species identified as S. mansoni calreticulin, is a good T- and B-cell antigen and represents a potential vaccine candidate.
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October 2000
Research Article|
October 01 2000
A SCHISTOSOMA MANSONI 62-KDA BAND IS IDENTIFIED AS AN IRRADIATED VACCINE T-CELL ANTIGEN AND CHARACTERIZED AS CALRETICULIN
Naglaa El Gengehi
;
Naglaa El Gengehi
Biomedical Research Centre for Infectious Diseases, the Egyptian Organization for Biological Products and Vaccines, Cairo, Egypt, and Department of Zoology, Faculty of Science, Cairo University, Cairo 12613, Egypt
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Rashika El Ridi
;
Rashika El Ridi
Biomedical Research Centre for Infectious Diseases, the Egyptian Organization for Biological Products and Vaccines, Cairo, Egypt, and Department of Zoology, Faculty of Science, Cairo University, Cairo 12613, Egypt
*To whom correspondence should be addressed; Department of Zoology, Faculty of Science, Cairo University, Cairo 12613, Egypt.
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Nagwa Abdel Tawab
;
Nagwa Abdel Tawab
Biomedical Research Centre for Infectious Diseases, the Egyptian Organization for Biological Products and Vaccines, Cairo, Egypt, and Department of Zoology, Faculty of Science, Cairo University, Cairo 12613, Egypt
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Maha El Demellawy
;
Maha El Demellawy
Biomedical Research Centre for Infectious Diseases, the Egyptian Organization for Biological Products and Vaccines, Cairo, Egypt, and Department of Zoology, Faculty of Science, Cairo University, Cairo 12613, Egypt
†Department of Biomedical Research, Genetic Engineering and Biotechnology Research Institute, Mobarak City for Scientific Research and Applied Technology, Alexandria, Egypt.
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Beverly L. Mangold
Beverly L. Mangold
Biomedical Research Centre for Infectious Diseases, the Egyptian Organization for Biological Products and Vaccines, Cairo, Egypt, and Department of Zoology, Faculty of Science, Cairo University, Cairo 12613, Egypt
§Naval Medical Research Institute, Bethesda, Maryland 20889.
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J Parasitol (2000) 86 (5): 993–1000.
Citation
Naglaa El Gengehi, Rashika El Ridi, Nagwa Abdel Tawab, Maha El Demellawy, Beverly L. Mangold; A SCHISTOSOMA MANSONI 62-KDA BAND IS IDENTIFIED AS AN IRRADIATED VACCINE T-CELL ANTIGEN AND CHARACTERIZED AS CALRETICULIN. J Parasitol 1 October 2000; 86 (5): 993–1000. doi: https://doi.org/10.1645/0022-3395(2000)086[0993:ASMKBI]2.0.CO;2
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