Intraspecific heterogeneity was demonstrated in the mini-exon gene localization from Leishmania (Viannia) panamensis and L. (Viannia) guyanensis. Different karyotypes were detected in human isolates circulating in endemic areas of Colombia. The presence of mini-exon gene sequences on chromosomes of different sizes, ranging from 370 to 800 kb in L. (V.) panamensis and from 500 to 800 kb in L. (V.) guyanensis, was observed and was neither strain nor species specific. In some cases, hybridization with 2 chromosomes in the same strain was observed. The variability of chromosomal localization of mini-exon gene sequences of these 2 species highlights the genetic variability of the Viannia subgenus and the potential utility of the mini-exon gene as a molecular epidemiologic marker.
Skip Nav Destination
Close
Article navigation
December 2000
Research Article|
December 01 2000
INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA
Raquel S. Pacheco
;
Raquel S. Pacheco
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
Search for other works by this author on:
Octavio Fernandes
;
Octavio Fernandes
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
*Department of Tropical Medicine, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, RJ, Brazil; and Department of Pathology, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, RJ, Brazil.
Search for other works by this author on:
Graciela Salinas
;
Graciela Salinas
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
†Centro Internacional de Investigaciones Medicas, CIDEIM, Cali, Colombia.
Search for other works by this author on:
Iris Segura
;
Iris Segura
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
†Centro Internacional de Investigaciones Medicas, CIDEIM, Cali, Colombia.
Search for other works by this author on:
Hooman Momen
;
Hooman Momen
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
Search for other works by this author on:
Wim Degrave
;
Wim Degrave
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
Search for other works by this author on:
Nancy G. Saravia
;
Nancy G. Saravia
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
†Centro Internacional de Investigaciones Medicas, CIDEIM, Cali, Colombia.
Search for other works by this author on:
David A. Campbell
David A. Campbell
Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, Fiocruz. Av. Brasil 4365, Manguinhos 21045-900, Rio de Janeiro, Brazil
‡Department of Microbiology and Immunology, University of California, Los Angeles, California 90024.
Search for other works by this author on:
J Parasitol (2000) 86 (6): 1250–1253.
Citation
Raquel S. Pacheco, Octavio Fernandes, Graciela Salinas, Iris Segura, Hooman Momen, Wim Degrave, Nancy G. Saravia, David A. Campbell; INTRASPECIFIC HETEROGENEITY IN THE MINI-EXON GENE LOCALIZATION OF LEISHMANIA (VIANNIA) PANAMENSIS AND LEISHMANIA (VIANNIA) GUYANENSIS FROM COLOMBIA. J Parasitol 1 December 2000; 86 (6): 1250–1253. doi: https://doi.org/10.1645/0022-3395(2000)086[1250:IHITME]2.0.CO;2
Download citation file:
Close
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your Institution
0
Views
0
Citations
Citing articles via
A New Isospora (Apicomplexa: Eimeriidae) from the Summer Tanager, Piranga rubra (Aves: Passeriformes: Cardinalidae), in Oklahoma
Chris T. McAllister, R. Scott Seville
First Documentation of Nematode Dispharynx sp. in Scaled Quail
Trey E. Johnson, Joshua G. Cross, Ryan S. Luna
Euschoengastia pipistrelli (Acari: Trombiculidae) from American Perimyotis, Perimyotis subflavus (Chiroptera: Vespertilionidae): Novel Stereoscopic and Scanning Electron Microscopy
Chris T. McAllister, Lance A. Durden, Stephen E. Greiman
A Helminth Survey of Northern Bobwhite Quail (Colinus virginianus) and Passerines in the Rolling Plains Ecoregion of Texas
Jessica L. Herzog, Shannon P. Lukashow-Moore, Matthew Z. Brym, Aravindan Kalyanasundaram, Ronald J. Kendall
Insect Immune Evasion by Dauer and Nondauer Entomopathogenic Nematodes
Nancy Lara-Reyes, J. Guillermo Jiménez-Cortés, Jorge Canales-Lazcano, Bernardo Franco, Indrikis Krams, Jorge Contreras-Garduño