Outbred goats were fed 105 Toxoplasma gondii oocysts and were monitored twice weekly for 8 wk for rectal temperature, clinical signs, parasitemia, and antibody levels by indirect fluorescence antibody test (IFAT), latex agglutination test (LAT), and indirect enzyme-linked immunosorbent assay (ELISA). After 8 wk, all goats were killed, and samples of heart, skeletal muscle, brain, lymph nodes, kidneys, and liver were bioassayed in mice. Anorexia, fever, and lethargy were observed from day 3 to day 7 postinfection (PI). Parasitemia was detected by bioassay in 50% of infected goats from day 7 to day 14 PI. Viable T. gondii organisms were isolated from all infected goats. Antibodies to T. gondii were detected in some animals on day 10 PI by IFAT and LAT and on day 14 PI by ELISA. The infected goats were seropositive on day 17 PI.
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April 2001
RESEARCH NOTE|
April 01 2001
Antibody Levels in Goats Fed Toxoplasma gondii Oocysts Available to Purchase
S. M. Nishi;
S. M. Nishi
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil, CEP 05508-000
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N. Kasai;
N. Kasai
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil, CEP 05508-000
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S. M. Gennari
S. M. Gennari
Department of Preventive Veterinary Medicine and Animal Health, Faculty of Veterinary Medicine, University of São Paulo, Av. Prof. Dr. Orlando Marques de Paiva, 87, São Paulo, SP, Brazil, CEP 05508-000
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J Parasitol (2001) 87 (2): 445–447.
Citation
S. M. Nishi, N. Kasai, S. M. Gennari; Antibody Levels in Goats Fed Toxoplasma gondii Oocysts. J Parasitol 1 April 2001; 87 (2): 445–447. doi: https://doi.org/10.1645/0022-3395(2001)087[0445:ALIGFT]2.0.CO;2
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