A phage display library was made starting from a cDNA library from the hematophagous human parasite Necator americanus. The cDNA library was transferred by polymerase chain reaction (PCR) cloning into phage display vectors (phagemids), using specially designed primers such that proteins would be expressed as fusions with the C-terminal part of the phage coat protein pVI. The vectors used are multicloning site variants of the original pDONG vectors described by Jespers et al. (1995). Electroporation of the ligation mixtures into electrocompetent Escherichia coli TG1 cells yielded 3 × 108 pG6A, 1.9 × 108 pG6B, and 1 × 108 pG6C transfectants for N. americanus. The final libraries consisted of a mix of equal numbers of insert-containing phages from the A, B, and C libraries. Selection of phages for binding to human collagen was performed. Four rounds of panning on human collagens I and III resulted in a significant enrichment of collagen-binding phages from the N. americanus libraries. PCR analysis revealed various insert lengths; however, sequence determination indicated that all phages contained the same protein, albeit with different poly-A tail lengths. The encoded protein itself is a 135-amino acid protein (15 kDa), with no apparent homology to any other known protein. Next the protein was recloned into E. coli using the pET-15b-vector. Upon isopropyl-1-thio-β-d-galactopyranoside induction, the recombinant protein, rNecH1, could be recovered by urea treatment from inclusion bodies. The rNecH1 protein binds to different collagens: human I > rat I > human III = calf skin I in a specific, dose-dependent, and saturable manner.
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June 2001
MOLECULAR-CELL BIOLOGY|
June 01 2001
Identification of a Collagen-Binding Protein from Necator americanus by Using a cDNA-Expression Phage Display Library Available to Purchase
A. VIAENE;
A. VIAENE
Laboratory for Thrombosis Research, IRC, KU Leuven Campus Kortrijk, E. Sabbelaan 53, B-8500 Kortrijk, Belgium
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A. CRAB;
A. CRAB
Laboratory for Thrombosis Research, IRC, KU Leuven Campus Kortrijk, E. Sabbelaan 53, B-8500 Kortrijk, Belgium
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M. MEIRING;
M. MEIRING
Laboratory for Thrombosis Research, IRC, KU Leuven Campus Kortrijk, E. Sabbelaan 53, B-8500 Kortrijk, Belgium
*Present address: Department of Haematology and Cell Biology, University of the Orange, Free State Bloemfontein, South Africa.
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D. PRITCHARD;
D. PRITCHARD
Laboratory for Thrombosis Research, IRC, KU Leuven Campus Kortrijk, E. Sabbelaan 53, B-8500 Kortrijk, Belgium
†The Boots Science Institute, School of Pharmaceutical Sciences, University of Nottingham, Nottingham, U.K.
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H. DECKMYN
H. DECKMYN
Laboratory for Thrombosis Research, IRC, KU Leuven Campus Kortrijk, E. Sabbelaan 53, B-8500 Kortrijk, Belgium
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J Parasitol (2001) 87 (3): 619–625.
Citation
A. VIAENE, A. CRAB, M. MEIRING, D. PRITCHARD, H. DECKMYN; Identification of a Collagen-Binding Protein from Necator americanus by Using a cDNA-Expression Phage Display Library. J Parasitol 1 June 2001; 87 (3): 619–625. doi: https://doi.org/10.1645/0022-3395(2001)087[0619:IOACBP]2.0.CO;2
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