DNA templates were extracted from isolates of Sarcocystis hominis-like cysts collected from cattle and water buffalo, as well as from Sarcocystis fusiformis cysts and Sarcocystis suihominis cysts. The 18S rRNA genes were amplified using DNA from a single cyst as the templates. Approximately 1,367–1,440 bp sequences were obtained. The sequence difference in isolates of Sarcocystis hominis-like cysts from water buffaloes, and isolates of S. hominis cysts from cattle were very low, only about 0.1%, much lower than the lowest value (1.7%) among different species. Combined with their morphological structure, these sequence data indicate that the 4 isolates from cattle and water buffalo might be the same species, i.e., S. hominis, suggesting that both cattle and water buffalo may serve as the intermediate hosts for this parasite. Apparently, this is the first report using a single cyst to do such work and is a useful way to distinguish the Sarcocystis cyst in an intermediate host that may be simultaneously infected by several different Sarcocystis species.
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August 2001
RESEARCH NOTES|
August 01 2001
Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) Cyst in Water Buffalo (Bubalus bubalis) Based on 18S rRNA Gene Sequences Available to Purchase
Zhao-Qing Yang;
Zhao-Qing Yang
bParasitology Department, Kunming Medical College, Kunming, Yunnan 650031 China
cLaboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
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Yang-Xian Zuo;
Yang-Xian Zuo
dTo whom correspondence should be addressed.
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Bo Ding;
Bo Ding
cLaboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
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Xin-Wen Chen;
Xin-Wen Chen
aBiology Department, Yunnan University, Kunming, Yunnan 650091 China
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Jing Luo;
Jing Luo
cLaboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
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Ya-Ping Zhang
Ya-Ping Zhang
cLaboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
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J Parasitol (2001) 87 (4): 934–937.
Citation
Zhao-Qing Yang, Yang-Xian Zuo, Bo Ding, Xin-Wen Chen, Jing Luo, Ya-Ping Zhang; Identification of Sarcocystis hominis-like (Protozoa: Sarcocystidae) Cyst in Water Buffalo (Bubalus bubalis) Based on 18S rRNA Gene Sequences. J Parasitol 1 August 2001; 87 (4): 934–937. doi: https://doi.org/10.1645/0022-3395(2001)087[0934:IOSHLP]2.0.CO;2
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