The indirect immunofluorescent antibody test (IFAT) and enzyme-linked immunosorbent assay (ELISA) were compared with blood culture for the detection of Trypanosoma cruzi infection in 83 raccoons (Procyon lotor) trapped in 4 counties of southeast Georgia. Both IFAT and ELISA detected 24 of 25 culture-positive samples (96% sensitivity). Cultures from 25 raccoons (30%) were positive for epimastigotes, whereas a total of 50 raccoons (60%) was seropositive by either the IFAT or ELISA. Forty-five of 83 serum samples (54%) were positive for anti-T. cruzi antibodies with the ELISA, and 47 were IFAT positive (57%). Forty-two of the 50 seropositive raccoons (84%) were seropositive by both tests. Endpoint titers of IFAT-positive samples were determined by testing doubling dilutions from 1:40 to 1:1280. High titers of 640 and 320 were observed for 4 raccoons trapped in 1 county (St. Catherines Island, Liberty County) and titers of 160 for 1–2 raccoons from each of the 4 counties sampled. IFAT titers and ELISA optical density values were positively correlated. Both serological tests have a high sensitivity and should be excellent tools for studying the prevalence of T. cruzi in wildlife populations.
COMPARISON OF SEROLOGICAL METHODS AND BLOOD CULTURE FOR DETECTION OF TRYPANOSOMA CRUZI INFECTION IN RACCOONS (PROCYON LOTOR)
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Michael J. Yabsley, Gayle Pittman Noblet, Oscar J. Pung; COMPARISON OF SEROLOGICAL METHODS AND BLOOD CULTURE FOR DETECTION OF TRYPANOSOMA CRUZI INFECTION IN RACCOONS (PROCYON LOTOR). J Parasitol 1 October 2001; 87 (5): 1155–1159. doi: https://doi.org/10.1645/0022-3395(2001)087[1155:COSMAB]2.0.CO;2
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