High-resolution typing of Toxoplasma gondii is essential to understand the effect of genetic differences among strains on the variation in disease manifestation and transmission patterns. Current typing methods discern 3 lineages with minimal within-lineage variation. Described here are 6 new variable loci. These loci, including a minisatellite and 5 microsatellites, were more polymorphic than allozymes, restriction fragment length polymorphisms, and sequence variation in introns. Most importantly, these loci revealed, for the first time, substantial within-lineage variation that was over 6-fold higher than that detected by other markers. Genotyping at these loci facilitates classification of isolates beyond the lineage level.
High-Resolution Typing of Toxoplasma gondii Using Microsatellite Loci
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C. R. Blackston, J. P. Dubey, E. Dotson, C. Su, P. Thulliez, D. Sibley, T. Lehmann; High-Resolution Typing of Toxoplasma gondii Using Microsatellite Loci. J Parasitol 1 December 2001; 87 (6): 1472–1475. doi: https://doi.org/10.1645/0022-3395(2001)087[1472:HRTOTG]2.0.CO;2
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