Neospora caninum was isolated from the brain of a naturally infected pregnant sheep by inoculation of immunodeficient mice with a homogenate of the brain tissue. The ewe showed no clinical signs. Tachyzoites were observed in the tissues of the nu/nu mice injected with the brain tissue homogenate and the diagnosis was confirmed by immunohistochemical staining with anti-N. caninum antibodies and by detecting N. caninum-specific DNA by polymerase chain reaction.
Isolation of Neospora caninum From the Brain of a Pregnant Sheep
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T. Koyama, Y. Kobayashi, Y. Omata, M. Yamada, H. Furuoka, R. Maeda, T. Matsui, A. Saito, T. Mikami; Isolation of Neospora caninum From the Brain of a Pregnant Sheep. J Parasitol 1 December 2001; 87 (6): 1486–1488. doi: https://doi.org/10.1645/0022-3395(2001)087[1486:IONCFT]2.0.CO;2
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