A non-isotopic in situ hybridization (ISH) method was developed for detection of Tetramicra brevifilum, a commercially important parasite in farmed turbot Scophthalmus maximus. The probe relies on sequences from the small-subunit rRNA gene (SSUrDNA) of Tetramicra brevifilum and was obtained by polymerase chain reaction then labeled with digoxigenin. The results obtained demonstrate that the probe hybridizes well with genomic DNA of the spores; thus, it is an effective method for detecting multiorgan infections of turbot by T. brevifilum.
Non-isotopic Detection of Tetramicra brevifilum (Microspora) DNA in Turbot Tissues
- Views Icon Views
- Share Icon Share
- Search Site
J. Leiro, R. Iglesias, F. M. Ubeira, M. L. Sanmartín; Non-isotopic Detection of Tetramicra brevifilum (Microspora) DNA in Turbot Tissues. J Parasitol 1 December 2001; 87 (6): 1488–1490. doi: https://doi.org/10.1645/0022-3395(2001)087[1488:NIDOTB]2.0.CO;2
Download citation file: