Epimastigote culture forms of different isolates of Trypanosoma cruzi from different mammal hosts, humans, and vectors were tested with FITC-conjugated peanut agglutinin lectin (PNA–FITC). The parasites maintained in axenic medium, liver infusion tryptose, were evaluated by flow cytometric analyses; whereas T. cruzi I (TcI), which is associated with the sylvatic transmission cycle, was labeled in high percentages with PNA (88–99.2%), T. cruzi II (TcII) (parasites associated with domiciliar cycle) and T. cruzi, zymodeme 3 (Tc/Z3) (also associated with the sylvatic cycle) were labeled in low percentages (TcII, 0–26% and Tc/Z3, 0–12.6%). It was demonstrated that it is possible to differentiate the 2 main T. cruzi subpopulations, TcI and TcII, using Arachis hypogaea. These results also showed a higher variability in TcII in terms of PNA binding.
TRYPANOSOMA CRUZI I AND TRYPANOSOMA CRUZI II: RECOGNITION OF SUGAR STRUCTURES BY ARACHIS HYPOGAEA (PEANUT AGGLUTININ) LECTIN
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C. A C. Araújo, C. B. Mello, A. M. Jansen; TRYPANOSOMA CRUZI I AND TRYPANOSOMA CRUZI II: RECOGNITION OF SUGAR STRUCTURES BY ARACHIS HYPOGAEA (PEANUT AGGLUTININ) LECTIN. J Parasitol 1 June 2002; 88 (3): 582–586. doi: https://doi.org/10.1645/0022-3395(2002)088[0582:TCIATC]2.0.CO;2
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