Resistance of Acanthamoeba castellanii cysts to disinfection agents, antimicrobial agents, heat, freeze–thawing, ultraviolet radiation (UV), γ irradiation, and cellulase were evaluated in vitro. Following exposure to different agents, the cysts were removed and cultured for A. castellanii trophozoites for 3–14 days. Solutions containing 20% isopropyl alcohol or 10% formalin effectively killed A. castellanii cysts. Hydrogen peroxide (3%, AOSept Disinfectant) effectively killed A. castellanii cysts after 4 hr of exposure. Polyhexamethylene biguanide (0.02%), clotrimazole (0.1%), or propamidine isethionate (Brolene) were effective in killing A. castellanii cysts in vitro. Acanthamoeba castellanii cysts were resistant to both 250 K rads of γ irradiation and 800 mJ/cm2 of UV irradiation. Excystment of trophozoites was accelerated after exposure to 10, 100, and, 1,000 units of cellulase. These results suggest that A. castellanii cysts benefit by enhanced survival because of their resistance to very harsh environmental conditions.
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June 2002
RESEARCH NOTES|
June 01 2002
Resistance of Acanthamoeba castellanii Cysts to Physical, Chemical, and Radiological Conditions
A. Aksozek;
A. Aksozek
aDepartment of Microbiology and Clinical Microbiology, Istanbul Faculty of Medicine, University of Istanbul, Capa/Istanbul, Turkey 34390
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K. McClellan;
K. McClellan
bDepartment of Ophthalmology, University of Sydney, G.P.O. Box 4337, Sydney, NSW 2001, Australia
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K. Howard;
K. Howard
cDepartment of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390-9057
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J. Y. Niederkorn;
J. Y. Niederkorn
cDepartment of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390-9057
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H. Alizadeh
H. Alizadeh
cDepartment of Ophthalmology, University of Texas Southwestern Medical Center at Dallas, Dallas, Texas 75390-9057
dTo whom correspondence should be addressed. [email protected]
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J Parasitol (2002) 88 (3): 621–623.
Citation
A. Aksozek, K. McClellan, K. Howard, J. Y. Niederkorn, H. Alizadeh; Resistance of Acanthamoeba castellanii Cysts to Physical, Chemical, and Radiological Conditions. J Parasitol 1 June 2002; 88 (3): 621–623. doi: https://doi.org/10.1645/0022-3395(2002)088[0621:ROACCT]2.0.CO;2
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