Laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. The vaccinated dogs were then challenged orally with 400 third-stage infective larvae (L3) of the canine hookworm, Ancylostoma caninum. The 3 A. caninum antigens selected were Ac-TMP, an adult-specific secreted tissue inhibitor of metalloproteases; Ac-AP, an adult-specific secreted factor Xa serine protease inhibitor anticoagulant; and Ac-ARR-1, a cathepsin D–like aspartic protease. Each of the 3 groups comprised 6 male beagles (8 ± 1 wk of age). A fourth group comprised control dogs injected with alum. All of the dogs vaccinated with Ac-TMP or Ac-APR-1 exhibited a vigorous antigen-specific antibody response, whereas only a single dog vaccinated with Ac-AP developed an antibody response. Dogs with circulating antibody responses exhibited 4.5–18% reduction in the numbers of adult hookworms recovered from the small intestines at necropsy, relative to alum-injected dogs. In contrast, there was a concomitant increase in the number of adult hookworms recovered from the colon. The increase in colonic hookworms was as high as 500%, relative to alum-injected dogs. Female adult hookworms were more likely to migrate into the colon than were males. Anti-enzyme and anti-enzyme inhibitor antibodies correlated with an alteration in adult hookworm habitat selection in the canine gastrointestinal tract.
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August 2002
IMMUNOLOGY|
August 01 2002
EFFECT OF VACCINATIONS WITH RECOMBINANT FUSION PROTEINS ON ANCYLOSTOMA CANINUM HABITAT SELECTION IN THE CANINE INTESTINE
Peter J. Hotez;
Peter J. Hotez
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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James Ashcom;
James Ashcom
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Zhan Bin;
Zhan Bin
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Jeffrey Bethony;
Jeffrey Bethony
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Angela Williamson;
Angela Williamson
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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John M. Hawdon;
John M. Hawdon
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Feng Jianjun;
Feng Jianjun
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Azra Dobardzic;
Azra Dobardzic
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Ivania Rizo;
Ivania Rizo
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Janelle Bolden;
Janelle Bolden
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Qun Jin;
Qun Jin
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Wang Yan;
Wang Yan
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Reshad Dobardzic;
Reshad Dobardzic
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Sophia Chung-Debose;
Sophia Chung-Debose
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Melissa Crowell;
Melissa Crowell
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Bennett Datu;
Bennett Datu
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Angela Delaney;
Angela Delaney
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Dilyan Dragonovski;
Dilyan Dragonovski
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Yang Jiang;
Yang Jiang
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Liu Yueyuan;
Liu Yueyuan
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Kashinath Ghosh;
Kashinath Ghosh
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Alex Loukas;
Alex Loukas
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Walter Brandt;
Walter Brandt
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Philip K. Russell;
Philip K. Russell
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
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Bernard C. Zook
Bernard C. Zook
Department of Microbiology and Tropical Medicine and Sabin Vaccine Institute, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037. [email protected]
* Department of Pathology, The George Washington University Medical Center, 2300 Eye Street NW, Washington, D.C. 20037
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J Parasitol (2002) 88 (4): 684–690.
Citation
Peter J. Hotez, James Ashcom, Zhan Bin, Jeffrey Bethony, Angela Williamson, John M. Hawdon, Feng Jianjun, Azra Dobardzic, Ivania Rizo, Janelle Bolden, Qun Jin, Wang Yan, Reshad Dobardzic, Sophia Chung-Debose, Melissa Crowell, Bennett Datu, Angela Delaney, Dilyan Dragonovski, Yang Jiang, Liu Yueyuan, Kashinath Ghosh, Alex Loukas, Walter Brandt, Philip K. Russell, Bernard C. Zook; EFFECT OF VACCINATIONS WITH RECOMBINANT FUSION PROTEINS ON ANCYLOSTOMA CANINUM HABITAT SELECTION IN THE CANINE INTESTINE. J Parasitol 1 August 2002; 88 (4): 684–690. doi: https://doi.org/10.1645/0022-3395(2002)088[0684:EOVWRF]2.0.CO;2
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