Laboratory dogs were vaccinated subcutaneously with 3 different recombinant fusion proteins, each precipitated with alum or calcium phosphate. The vaccinated dogs were then challenged orally with 400 third-stage infective larvae (L3) of the canine hookworm, Ancylostoma caninum. The 3 A. caninum antigens selected were Ac-TMP, an adult-specific secreted tissue inhibitor of metalloproteases; Ac-AP, an adult-specific secreted factor Xa serine protease inhibitor anticoagulant; and Ac-ARR-1, a cathepsin D–like aspartic protease. Each of the 3 groups comprised 6 male beagles (8 ± 1 wk of age). A fourth group comprised control dogs injected with alum. All of the dogs vaccinated with Ac-TMP or Ac-APR-1 exhibited a vigorous antigen-specific antibody response, whereas only a single dog vaccinated with Ac-AP developed an antibody response. Dogs with circulating antibody responses exhibited 4.5–18% reduction in the numbers of adult hookworms recovered from the small intestines at necropsy, relative to alum-injected dogs. In contrast, there was a concomitant increase in the number of adult hookworms recovered from the colon. The increase in colonic hookworms was as high as 500%, relative to alum-injected dogs. Female adult hookworms were more likely to migrate into the colon than were males. Anti-enzyme and anti-enzyme inhibitor antibodies correlated with an alteration in adult hookworm habitat selection in the canine gastrointestinal tract.
EFFECT OF VACCINATIONS WITH RECOMBINANT FUSION PROTEINS ON ANCYLOSTOMA CANINUM HABITAT SELECTION IN THE CANINE INTESTINE
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Peter J. Hotez, James Ashcom, Zhan Bin, Jeffrey Bethony, Angela Williamson, John M. Hawdon, Feng Jianjun, Azra Dobardzic, Ivania Rizo, Janelle Bolden, Qun Jin, Wang Yan, Reshad Dobardzic, Sophia Chung-Debose, Melissa Crowell, Bennett Datu, Angela Delaney, Dilyan Dragonovski, Yang Jiang, Liu Yueyuan, Kashinath Ghosh, Alex Loukas, Walter Brandt, Philip K. Russell, Bernard C. Zook; EFFECT OF VACCINATIONS WITH RECOMBINANT FUSION PROTEINS ON ANCYLOSTOMA CANINUM HABITAT SELECTION IN THE CANINE INTESTINE. J Parasitol 1 August 2002; 88 (4): 684–690. doi: https://doi.org/10.1645/0022-3395(2002)088[0684:EOVWRF]2.0.CO;2
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