Fetal loss and vertical transmission of Neospora caninum were evaluated in outbred Quackenbush (Qs) mice with respect to dose of parasites, N. caninum isolate, and route of injection. Mice were infected with NC-Liverpool or NC-SweB1 at day 5 or 8 of pregnancy with doses of 104, 106, or 107 parasites, through either a subcutaneous or intraperitoneal injection. Polymerase chain reaction was used to detect N. caninum in the brains of offspring, and enzyme-linked immunosorbent assay was used to analyze the maternal immune response. Vertical transmission occurred in mice given 106 NC-Liverpool at day 5 during gestation, and a significant (P < 0.05) maternal antibody response was observed in mice infected with NC-Liverpool or NC-SweB1 at days 5 and 8 of gestation. This study shows that outbred Qs mice are a useful model for the study of vertical transmission associated with N. caninum, as they display less clinical disease and pathogenesis than inbred mice and have large litters, which is advantageous when studying maternal transmission.
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August 2002
Research Article|
August 01 2002
CHARACTERIZATION OF AN OUTBRED PREGNANT MOUSE MODEL OF NEOSPORA CANINUM INFECTION
Helen E. Quinn;
Helen E. Quinn
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology Sydney, Westbourne Street, Gore Hill, New South Wales 2065, Australia. j.ellis@uts.edu.au
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Catherine M. D. Miller;
Catherine M. D. Miller
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology Sydney, Westbourne Street, Gore Hill, New South Wales 2065, Australia. j.ellis@uts.edu.au
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Cheryl Ryce;
Cheryl Ryce
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology Sydney, Westbourne Street, Gore Hill, New South Wales 2065, Australia. j.ellis@uts.edu.au
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Peter A. Windsor;
Peter A. Windsor
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology Sydney, Westbourne Street, Gore Hill, New South Wales 2065, Australia. j.ellis@uts.edu.au
* New South Wales Agriculture, Elizabeth Macarthur Agricultural Institute, Camden, New South Wales 2570, Australia
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John T. Ellis
John T. Ellis
Molecular Parasitology Unit, Department of Cell and Molecular Biology, University of Technology Sydney, Westbourne Street, Gore Hill, New South Wales 2065, Australia. j.ellis@uts.edu.au
† Author for correspondence.
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J Parasitol (2002) 88 (4): 691–696.
Citation
Helen E. Quinn, Catherine M. D. Miller, Cheryl Ryce, Peter A. Windsor, John T. Ellis; CHARACTERIZATION OF AN OUTBRED PREGNANT MOUSE MODEL OF NEOSPORA CANINUM INFECTION. J Parasitol 1 August 2002; 88 (4): 691–696. doi: https://doi.org/10.1645/0022-3395(2002)088[0691:COAOPM]2.0.CO;2
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