Aotus lemurinus griseimembra is considered one of the best nonhuman primate species for malarial studies because of its susceptibility to infection by Plasmodium falciparum asexual blood stages. However, reproducible transmission of infective P. falciparum sporozoites by mosquito inoculation has been difficult to achieve even in splenectomized monkeys. Characterization of an Aotus–P. falciparum cyclical transmission model has become a top priority as a result of the significant progress toward the development of preerythrocytic malaria vaccines. Herein, we describe a reproducible model developed using intact A. lemurinus griseimembra monkeys intravenously inoculated with sporozoites from a monkey-adapted P. falciparum (Santa Lucia) strain and a wild Falciparum-Cali-Colombia-4 (FCC-4) strain. Sporozoites were obtained by salivary gland dissection of laboratory-reared Anopheles albimanus mosquitoes. Parasitemia was monitored by thick-smear microscopy, parasite lactate dehydrogenase (pLDH) determination, and mosquito xenodiagnosis. The last method proved to be the most sensitive method for monitoring parasitemias. Infection with the Santa Lucia strain showed a mean prepatent period of 16 days (range 6–21 days), whereas infection with the wild FCC-4 strain resulted in a 24-day prepatent period. Mean peak parasite density was approximately 900 parasites/μl for both parasite strains. The prepatent period, the peak of parasitemia, and the duration of patency were independent of the size of the sporozoite inoculum and the presence of spleen in the host. This model is being successfully used to test the protective efficacy of P. falciparum preerythrocytic vaccine candidates.
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August 2002
LIFE CYCLES-SURVEY|
August 01 2002
REPRODUCIBLE INFECTION OF INTACT AOTUS LEMURINUS GRISEIMEMBRA MONKEYS BY PLASMODIUM FALCIPARUM SPOROZOITE INOCULATION
Judith Constanza Zapata;
Judith Constanza Zapata
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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Blanca Liliana Perlaza;
Blanca Liliana Perlaza
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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Silvia Hurtado;
Silvia Hurtado
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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Gustavo E. Quintero;
Gustavo E. Quintero
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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Diana Jurado;
Diana Jurado
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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Iveth González;
Iveth González
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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Pierre Druilhe;
Pierre Druilhe
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
* Biomedical Parasitology Unit, Pasteur Institute, 75724 Paris, France
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Myriam Arévalo-Herrera;
Myriam Arévalo-Herrera
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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Sócrates Herrera
Sócrates Herrera
Immunology Institute, University of Valle, AA 25574 Cali, Colombia. [email protected]
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J Parasitol (2002) 88 (4): 723–729.
Citation
Judith Constanza Zapata, Blanca Liliana Perlaza, Silvia Hurtado, Gustavo E. Quintero, Diana Jurado, Iveth González, Pierre Druilhe, Myriam Arévalo-Herrera, Sócrates Herrera; REPRODUCIBLE INFECTION OF INTACT AOTUS LEMURINUS GRISEIMEMBRA MONKEYS BY PLASMODIUM FALCIPARUM SPOROZOITE INOCULATION. J Parasitol 1 August 2002; 88 (4): 723–729. doi: https://doi.org/10.1645/0022-3395(2002)088[0723:RIOIAL]2.0.CO;2
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