Cats are pivotal in the transmission of Toxoplasma gondii. To develop a sensitive and specific serodiagnostic method for feline toxoplasmosis, surface antigen 2 (SAG2) of T. gondii was expressed in Escherichia coli and its diagnostic potential evaluated in an enzyme-linked immunosorbent assay (ELISA). The ELISA with recombinant SAG2 (rSAG2) was able to differentiate very clearly between sera from cats experimentally infected with T. gondii and sera from normal cats. Serum samples collected from domestic cats in Japan were investigated by the ELISA, and the results were compared with those of a commercially available latex agglutination test (LAT) kit. Of the 192 samples screened, 42 (21.9%) were positive by ELISA. Among the 42 ELISA-positive samples, 39 were positive by LAT. There was a significant correlation between ELISA and LAT titers. All the 150 ELISA-negative samples were negative by LAT. These results indicate that the ELISA with rSAG2 expressed in E. coli should be a useful method for detection of T. gondii infection in cats.
Skip Nav Destination
Article navigation
August 2002
Research Article|
August 01 2002
Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats
X. Huang;
X. Huang
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
X. Xuan;
X. Xuan
aTo whom correspondence should be addressed
Search for other works by this author on:
E. N. Kimbita;
E. N. Kimbita
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
B. Battur;
B. Battur
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
T. Miyazawa;
T. Miyazawa
cResearch Institute for Microbial Diseases, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan
Search for other works by this author on:
S. Fukumoto;
S. Fukumoto
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
M. Mishima;
M. Mishima
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
L. H. Makala;
L. H. Makala
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
H. Suzuki;
H. Suzuki
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
C. Sugimoto;
C. Sugimoto
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
H. Nagasawa;
H. Nagasawa
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
K. Fujisaki;
K. Fujisaki
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
T. Mikami;
T. Mikami
dCollege of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-8510, Japan. gen@obihiro.ac.jp
Search for other works by this author on:
I. Igarashi
I. Igarashi
bNational Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan
Search for other works by this author on:
J Parasitol (2002) 88 (4): 804–807.
Citation
X. Huang, X. Xuan, E. N. Kimbita, B. Battur, T. Miyazawa, S. Fukumoto, M. Mishima, L. H. Makala, H. Suzuki, C. Sugimoto, H. Nagasawa, K. Fujisaki, T. Mikami, I. Igarashi; Development and Evaluation of an Enzyme-Linked Immunosorbent Assay with Recombinant SAG2 for Diagnosis of Toxoplasma gondii Infection in Cats. J Parasitol 1 August 2002; 88 (4): 804–807. doi: https://doi.org/10.1645/0022-3395(2002)088[0804:DAEOAE]2.0.CO;2
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your Institution
8
Views
0
Citations
Citing articles via
CERCARIAL LONGEVITY AND INFECTIVITY OF BOLBOPHORUS DAMNIFICUS, WITH NOTES ON METACERCARIAL PERSISTENCE AND SITE SPECIFICITY IN CHANNEL AND HYBRID CATFISH
Mackenzie A. Gunn, Peter J. Allen, T. Graham Rosser, David J. Wise, Matt J. Griffin
DETECTION OF SPLENDIDOFILARIA SP. (ONCHOCERCIDAE:SPLENDIDOFILARIINAE) MICROFILARIA WITHIN ALASKAN GROUND-DWELLING BIRDS IN THE GROUSE SUBFAMILY TETRAONINAE USING TAQMAN PROBE-BASED REAL-TIME PCR
Stephen E. Greiman, Robert E. Wilson, Briana Sesmundo, Jack Reakoff, Sarah A. Sonsthagen
OCCURRENCE OF EUSTRONGYLIDES EXCISUS (NEMATODA:DIOCTOPHYMATIDAE) IN EUROPEAN PERCH (PERCA FLUVIATILIS) AND GREAT CORMORANT (PHALACROCORAX CARBO) IN LAKE ANNONE, NORTHERN ITALY
Aurora Rusconi, Paola Prati, Roldano Bragoni, Michele Castelli, Umberto Postiglione, Sara Rigamonti, Davide Sassera, Emanuela Olivieri
REVIEW OF THE ROLE OF PARASITIC NEMATODE EXCRETORY/SECRETORY PROTEINS IN HOST IMMUNOMODULATION
Ogadinma K. Okakpu, Adler R. Dillman
REVIEW OF DIOCTOPHYME RENALE: ETIOLOGY, MORPHOLOGY, BIOLOGY, ECOEPIDEMIOLOGY, PATHOGENESIS, SYMPTOMATOLOGY, DIAGNOSIS, TREATMENT, AND PREVENTION
Zully Hernández Russo, Jorge Cárdenas Callirgos, Alberto García-Ayachi, Eric J. Wetzel
