The effect of oryzalin on excystation and metacystic development of Entamoeba invadens strain IP-1 was examined by transfer of cysts to a growth medium containing the drug. Excystation, which was assessed by counting the number of metacystic amoebae after induction of excystation, was inhibited by oryzalin in a concentration-dependent manner. Metacystic development, which was determined by the number of nuclei in metacystic amoebae, was also inhibited by oryzalin because the percentage of 4-nucleate amoebae at day 1 remained unchanged at day 3. The addition of oryzalin after the induction of excystation decreased the number of metacystic amoebae, compared with control cultures. When cysts were incubated for 1 day in growth medium plus oryzalin, little increase in the number of metacystic amoebae was observed after removal of the drug. Excystation and metacystic development were further inhibited when the cysts were incubated for 30 min in encystation medium containing oryzalin before transfer to growth medium with the drug. When cysts were incubated for 30 min in encystation medium before transfer to growth medium without the drug, metacystic amoebae decreased in number. Pretreatment of cysts with oryzalin for 30 min in phosphate-buffered saline markedly reduced viability and prevented excystation in growth medium with or without the drug. The results indicate that oryzalin inhibits excystation and metacystic development of E. invadens, suggesting that it may be an inhibitor of Entamoeba infection.
Skip Nav Destination
Article navigation
October 2002
THERAPEUTICS-DIAGNOSTICS|
October 01 2002
INHIBITION OF EXCYSTATION AND METACYSTIC DEVELOPMENT OF ENTAMOEBA INVADENS BY THE DINITROANILINE HERBICIDE ORYZALIN Available to Purchase
Asao Makioka;
Asao Makioka
Department of Tropical Medicine, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. [email protected]
Search for other works by this author on:
Masahiro Kumagai;
Masahiro Kumagai
Department of Tropical Medicine, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. [email protected]
Search for other works by this author on:
Seiki Kobayashi;
Seiki Kobayashi
Department of Tropical Medicine, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. [email protected]
* Department of Tropical Medicine and Parasitology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Search for other works by this author on:
Tsutomu Takeuchi
Tsutomu Takeuchi
Department of Tropical Medicine, Jikei University School of Medicine, 3-25-8 Nishi-shinbashi, Minato-ku, Tokyo 105-8461, Japan. [email protected]
* Department of Tropical Medicine and Parasitology, Keio University School of Medicine, 35 Shinanomachi, Shinjuku-ku, Tokyo 160-8582, Japan.
Search for other works by this author on:
J Parasitol (2002) 88 (5): 994–999.
Citation
Asao Makioka, Masahiro Kumagai, Seiki Kobayashi, Tsutomu Takeuchi; INHIBITION OF EXCYSTATION AND METACYSTIC DEVELOPMENT OF ENTAMOEBA INVADENS BY THE DINITROANILINE HERBICIDE ORYZALIN. J Parasitol 1 October 2002; 88 (5): 994–999. doi: https://doi.org/10.1645/0022-3395(2002)088[0994:IOEAMD]2.0.CO;2
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
HAPLOTYPE DIVERSITY IN ENDOSYMBIOTIC BACTERIA FOLLOWING A HOST SWITCH BY PARASITIC LICE
Bret M. Boyd, Niyomi House, Ariel C. Toloza, David L. Reed
DESCRIPTION, REDESCRIPTION, AND LIFE CYCLES OF CLOACITREMA KURISI N. SP. AND CLOACITREMA MICHIGANENSIS (TREMATODA: DIGENEA: PHILOPHTHALMIDAE) FROM THE CALIFORNIA HORN SNAIL, CERITHIDEOPSIS CALIFORNICA (GASTROPODA: POTAMIDIDAE)
Alexandria P. Nelson, Daniel C. G. Metz, Ryan F. Hechinger
A NEW SPECIES OF HALIPEGUS (TREMATODA: DEROGENIDAE: HALIPEGINAE) IN LITHOBATES SPECTABILIS (ANURA: RANIDAE) FROM RESERVA DE LA BIOSFERA BARRANCA DE METZTITLAN, HIDALGO, MEXICO
Edgar S. Ramírez-Cruz, Griselda Pulido-Flores, Scott Monks, Elizabeth A. Martínez-Salazar
A SYSTEMATIC REVISION OF KROYERIA (COPEPODA: SIPHONOSTOMATOIDA): FIVE NEW SPECIES, 11 REDESCRIPTIONS, AND A MORPHOLOGICAL PHYLOGENETIC ANALYSIS OF THE GENUS
Gregory B. Deets, Geoffrey A. Boxshall, James P. Bernot
MOLECULAR SURVEY OF HAEMOSPORIDIAN PARASITES IN HAWKS, FALCONS, AND OWLS (ACCIPITRIFORMES, FALCONIFORMES, STRIGIFORMES) FROM MINNESOTA AND NORTH DAKOTA, WITH REMARKS ON THE PHYLOGENETIC RELATIONSHIPS OF HAEMOSPORIDIANS IN NORTH AMERICAN RAPTORS
Jeffrey A. Bell, Timothy G. Driscoll, Tyler J. Achatz, Jakson R. Martens, Jefferson A. Vaughan