The effect of moist heat and several disinfectants on Sarcocystis neurona sporocysts was investigated. Sporocysts (4 million) were suspended in water and heated to 50, 55, 60, 65, and 70 C for various times and were then bioassayed in interferon gamma gene knockout (KO) mice. Sporocysts heated to 50 C for 60 min and 55 C for 5 min were infective to KO mice, whereas sporocysts heated to 55 C for 15 min and 60 C or more for 1 min were rendered noninfective to mice. Treatment with bleach (10, 20, and 100%), 2% chlorhexidine, 1% betadine, 5% o-benzyl-p-chlorophenol, 12.56% phenol, 6% benzyl ammonium chloride, and 10% formalin was not effective in killing sporocysts. Treatment with undiluted ammonium hydroxide (29.5% ammonia) for 1 hr killed sporocysts, but treatment with a 10-fold dilution (2.95% ammonia) for 6 hr did not kill sporocysts. These data indicate that heat treatment is the most effective means of killing S. neurona sporocysts in the horse feed or in the environment.
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December 2002
Research Article|
December 01 2002
Effects of High Temperature and Disinfectants on the Viability of Sarcocystis neurona Sporocysts
J. P. Dubey;
J. P. Dubey
aParasite Biology, Epidemiology and Systematics Laboratory, Building 1001, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705-2350
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W. J. Saville;
W. J. Saville
bDepartment of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, 1900 Coffey Road, Columbus, Ohio 43210-1092
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C. Sreekumar;
C. Sreekumar
aParasite Biology, Epidemiology and Systematics Laboratory, Building 1001, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705-2350
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S. K. Shen;
S. K. Shen
aParasite Biology, Epidemiology and Systematics Laboratory, Building 1001, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705-2350
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D. S. Lindsay;
D. S. Lindsay
cCenter for Molecular and Infectious Diseases, Department of Biomedical Sciences and Pathobiology, Virginia-Maryland Regional College of Veterinary Medicine, 1410 Prices Fork Road, Virginia-Tech, Virginia 24061-0342
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H. F. Pena;
H. F. Pena
dFaculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando marques de paiva, 87, CEP 05508, São Paulo, Brazil
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M. C. Vianna;
M. C. Vianna
dFaculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando marques de paiva, 87, CEP 05508, São Paulo, Brazil
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S. M. Gennari;
S. M. Gennari
dFaculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo, Av. Prof. Dr. Orlando marques de paiva, 87, CEP 05508, São Paulo, Brazil
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S. M. Reed
S. M. Reed
eDepartment of Large Animal Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-1092. jdubey@anri.barc.usda.gov
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J Parasitol (2002) 88 (6): 1252–1254.
Citation
J. P. Dubey, W. J. Saville, C. Sreekumar, S. K. Shen, D. S. Lindsay, H. F. Pena, M. C. Vianna, S. M. Gennari, S. M. Reed; Effects of High Temperature and Disinfectants on the Viability of Sarcocystis neurona Sporocysts. J Parasitol 1 December 2002; 88 (6): 1252–1254. doi: https://doi.org/10.1645/0022-3395(2002)088[1252:EOHTAD]2.0.CO;2
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