A polymerase chain reaction–based restriction fragment length polymorphism (RFLP) approach is used to examine Sarcocystis cruzi–like taxa from the atypical intermediate host, water buffalo, in Yunnan, People's Republic of China. The loci examined lie within the 18S rRNA gene. A total of 15 water buffalo isolates are compared with those of 10 S. cruzi from cattle. RFLP patterns for the S. cruzi isolates from cattle and the S. cruzi–like taxon from water buffalo are found to be identical with all the 12 restriction enzymes used. Interpopulation variation between samples from Kunming and Gengma (Yunnan) is found to be undetectable at these loci for both S. cruzi and the S. cruzi–like taxon. But RFLPs are found between the S. cruzi taxa and S. suihominis from pigs at the same study sites. These findings support the hypothesis that S. cruzi is able to use the water buffalo as an intermediate host and is not restricted to cattle as was previously supposed.
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December 2002
RESEARCH NOTES|
December 01 2002
A PCR-Based RFLP Analysis of Sarcocystis cruzi (Protozoa: Sarcocystidae) in Yunnan Province, PR China, Reveals the Water Buffalo (Bubalus bubalis) as a Natural Intermediate Host
Qing-Qing Li;
Qing-Qing Li
bParasitology Department, Kunming Medical College, Kunming, Yunnan, People's Republic of China 650031
cLaboratory of Molecular Evolution and Genome Diversity, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People's Republic of China 650223
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Zhao-Qing Yang;
Zhao-Qing Yang
bParasitology Department, Kunming Medical College, Kunming, Yunnan, People's Republic of China 650031
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Yang-Xian Zuo;
Yang-Xian Zuo
aBiology Department, Yunnan University, Kunming, Yunnan, People's Republic of China 650091
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S. W. Attwood;
S. W. Attwood
eZoology Department, The Natural History Museum, Cromwell Road, London SW7 5BD, U.K
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Xin-Wen Chen;
Xin-Wen Chen
aBiology Department, Yunnan University, Kunming, Yunnan, People's Republic of China 650091
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Ya-Ping Zhang
Ya-Ping Zhang
cLaboratory of Molecular Evolution and Genome Diversity, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, People's Republic of China 650223
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J Parasitol (2002) 88 (6): 1259–1261.
Citation
Qing-Qing Li, Zhao-Qing Yang, Yang-Xian Zuo, S. W. Attwood, Xin-Wen Chen, Ya-Ping Zhang; A PCR-Based RFLP Analysis of Sarcocystis cruzi (Protozoa: Sarcocystidae) in Yunnan Province, PR China, Reveals the Water Buffalo (Bubalus bubalis) as a Natural Intermediate Host. J Parasitol 1 December 2002; 88 (6): 1259–1261. doi: https://doi.org/10.1645/0022-3395(2002)088[1259:APBRAO]2.0.CO;2
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