Monoclonal antibodies (mAbs) were produced against the proteins of advanced third-stage larvae (AdL3) of Gnathostoma nipponicum. Six mAbs (Gn2C3, Gn2H3, Gn4C3, Gn4E9, Gn5H1, and Gn10B7) were obtained as determined by enzyme-linked immunosorbent assay (ELISA). Gn4E9 and Gn5H1 seemed to be genus-specific, as they did not cross-react with Anisakis sp., Dirofilaria immitis, Gongylonema pulchrum, Toxocara canis, Trichinella sp., Trichuris vulpis, Metagonimus sp., or Spirometra erinaceieuropaei by ELISA. Immunohistochemistry showed that Gn2C3, Gn4E9, and Gn5H1 reacted strongly with the central esophagus; Gn2H3 reacted with cuticle, muscle, intestine, and the cervical sac; and Gn4C3 and Gn10B7 reacted with cuticle, muscle, esophagus, intestine, and the cervical sac of AdL3. In Western blotting analysis, Gn2C3, Gn4E9, and Gn5H1 reacted to 60-, 53-, 46-, and 41-kDa proteins; Gn4C3 reacted to the AdL3 protein of G. nipponicum (>42 kDa). Moreover, proteins purified using a mAb Gn4E9 immunoprecipitation method (sizes 60-, 53-, 46-, and 41-kDa) were used as antigens in ELISAs. A significant difference (P < 0.01) was shown between mouse sera infected with G. nipponicum and sera infected with Trichnella sp. or not infected. These results provide a rationale for evaluating esophageal proteins for the development of diagnostic methods for detecting G. nipponicum or Gnathostoma sp. infections.
Skip Nav Destination
Article navigation
February 2003
RESEARCH NOTES|
February 01 2003
Characterization of Monoclonal Antibodies Against Gnathostoma nipponicum
H. Ikadai;
H. Ikadai
aDepartment of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan
Search for other works by this author on:
T. Fujii;
T. Fujii
aDepartment of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan
Search for other works by this author on:
T. Nagai;
T. Nagai
aDepartment of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan
Search for other works by this author on:
K. Yoshioka;
K. Yoshioka
bDepartment of Veterinary Anatomy, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan
Search for other works by this author on:
J. Nagasao;
J. Nagasao
bDepartment of Veterinary Anatomy, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan
Search for other works by this author on:
N. Kudo;
N. Kudo
aDepartment of Veterinary Parasitology, School of Veterinary Medicine and Animal Sciences, Kitasato University, Towada, Aomori 034-8628, Japan
Search for other works by this author on:
T. Oyamada
T. Oyamada
cTo whom correspondence should be addressed. oyamada@vmas.kitasato-u.ac.jp
Search for other works by this author on:
J Parasitol (2003) 89 (1): 180–183.
Citation
H. Ikadai, T. Fujii, T. Nagai, K. Yoshioka, J. Nagasao, N. Kudo, T. Oyamada; Characterization of Monoclonal Antibodies Against Gnathostoma nipponicum. J Parasitol 1 February 2003; 89 (1): 180–183. doi: https://doi.org/10.1645/0022-3395(2003)089[0180:COMAAG]2.0.CO;2
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
POPULATION STRUCTURE AND BARTONELLA QUINTANA IN HEAD AND BODY LICE IN POKHARA, NEPAL (ANOPLURA: PEDICULIDAE)
Shreekanta S. Poudel, Jefferson A. Vaughan
PALEOEPIDEMIOLOGY OF DIPHYLLOBOTHRIOSIS: CULTURAL AND ENVIRONMENTAL FACTORS AFFECTING ADENOCEPHALUS INTENSITY AND PREVALENCE
Karl J. Reinhard, Bernardo Arriaza, William Alexander Avery, Jane Buikstra, Morgana Camacho, Elizabeth Goodman, John Obafunwa, Bruce Owen, Isabel Teixeira-Santos
TAXONOMIC REASSESSMENT AND MORPHOLOGICAL REDESCRIPTION OF NEMATOSTRIGEA SERPENS ANNULATA (DIGENEA: STRIGEIDAE) FROM OSPREY IN NORTH AMERICA
Bharani Gudla, Sarah A. Orlofske, Sara V. Brant, Vasyl V. Tkach, Shelli Dubay, Lauren Holtz, Tyler J. Achatz
PRESCRIBING PATTERNS AND THE USE OF ANTHELMINTIC DRUGS IN COLOMBIAN PATIENTS: A CROSS-SECTIONAL STUDY
Maria Camila Montes-Montoya, Andrés Gaviria-Mendoza, Maria Mónica Murillo-Muñoz, Jaime Andrés Cardona-Ospina, Jorge Enrique Machado-Alba
MOLECULAR CHARACTERIZATION OF ORNITHOSTRONGYLUS QUADRIRADIATUS ISOLATED FROM A RACING PIGEON (COLUMBA LIVIA DOMESTICA)
Aleksandra Ledwoń, Ines Szotowska, Izabella Dolka, Piotr Szeleszczuk