Mesocestoides corti is a suitable model for studying cestode development because of its ability to reproduce asexually and segment in vitro. The cultured parasite is also capable of sexual differentiation and, probably, reproduction. To establish conditions that increase the efficiency of in vitro M. corti larvae (tetrathyridia) segmentation, we tested the effects of an inducing agent and some physical parameters in cultures. We found that a 5% CO2–95% N2 gas phase, an incubation temperature of 39 C (instead of 37 C), and a 24-hr pretreatment with trypsin (105 BAEE/ml, BAEE = Nα-benzoil-l-arginine ethyl ester unit of trypsin activity) in Roswell Park Memorial Institute (RPMI) 1640 medium supplemented with 20% fetal bovine serum (FBS) are able to increase individually or synergistically the segmentation rate of tetrathyridia. A segmentation rate of up to 100% was achieved on day 4 of culture, when all these conditions were used simultaneously, in comparison with an average rate of 40% obtained not before day 11 in cultures without any inducing treatment. Fetal bovine serum is essential for segmentation, and a concentration of 20% was established as the standard for induction.
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February 2003
FUNCTIONAL MORPHOLOGY|
February 01 2003
IN VITRO SEGMENTATION INDUCTION OF MESOCESTOIDES CORTI (CESTODA) TETRATHYRIDIA Available to Purchase
Melissa M. Markoski;
Melissa M. Markoski
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. [email protected]
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Cristiano V. Bizarro;
Cristiano V. Bizarro
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. [email protected]
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Sandra Farias;
Sandra Farias
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. [email protected]
* Departamento de Fisiologia, ICBS, Universidade Federal do Rio Grande do Sul, 90050-170 Porto Alegre, RS, Brazil
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Ingrid Espinoza;
Ingrid Espinoza
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. [email protected]
† Laboratorio de Biologia Molecular, ICBM, Facultad de Medicina, Universidad de Chile, 6530499 Santiago, Chile
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Norbel Galanti;
Norbel Galanti
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. [email protected]
† Laboratorio de Biologia Molecular, ICBM, Facultad de Medicina, Universidad de Chile, 6530499 Santiago, Chile
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Arnaldo Zaha;
Arnaldo Zaha
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. [email protected]
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Henrique B. Ferreira
Henrique B. Ferreira
Laboratório de Biologia Molecular de Cestódeos, Centro de Biotecnologia do Estado do Rio Grande do Sul, Universidade Federal do Rio Grande do Sul, 91501-970 Porto Alegre, RS, Brazil. [email protected]
‡ To whom correspondence should be addressed. Centro de Biotecnologia/UFRGS, Av. Bento Gonçalves, 9500, Prédio 43-421, Lab. 206, 91501-970 Porto Alegre, RS, Brazil
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J Parasitol (2003) 89 (1): 27–34.
Citation
Melissa M. Markoski, Cristiano V. Bizarro, Sandra Farias, Ingrid Espinoza, Norbel Galanti, Arnaldo Zaha, Henrique B. Ferreira; IN VITRO SEGMENTATION INDUCTION OF MESOCESTOIDES CORTI (CESTODA) TETRATHYRIDIA. J Parasitol 1 February 2003; 89 (1): 27–34. doi: https://doi.org/10.1645/0022-3395(2003)089[0027:IVSIOM]2.0.CO;2
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