Cross-reactions between Leishmania braziliensis and Trypanosoma cruzi caused by common antigenic determinants hinder the specific diagnosis of cutaneous and mucocutaneous leishmaniasis (CL and MCL). Therefore, the usefulness of the 70-kDa heat shock protein (Hsp70) from L. braziliensis for differential serodiagnosis was investigated. The single-copy gene encoding Hsp70, consisting of 663 amino acids, was isolated from a genomic DNA library. The antigenicity data show that Hsp70 is an immunodominant antigen highly recognized (84%) by sera of patients with CL and MCL and to a lesser extent by chagasic patients (18.75%). Antigenic mapping of the 5 overlapping fragments into which the protein was split showed that the main antigenic determinants are located in the carboxy-terminal end. The linear antigenic determinants that show cross-reactions with chagasic sera are located in the fragment rLb70(352–518). The carboxy-terminal fragment rLb70(513–663) presents 70% sensitivity and 100% specificity, so it could be a potential candidate for specific serodiagnosis of CL and MCL caused by L. braziliensis.
CLONING AND CHARACTERIZATION OF THE LEISHMANIA (VIANNIA) BRAZILIENSIS HSP70 GENE. DIAGNOSTIC USE OF THE C-TERMINAL FRAGMENT rLb70(513–663)
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Ada I. Zurita, Javier Rodríguez, José E. Piñero, Rosa Pacheco, Emma Carmelo, Antonio del Castillo, Basilio Valladares; CLONING AND CHARACTERIZATION OF THE LEISHMANIA (VIANNIA) BRAZILIENSIS HSP70 GENE. DIAGNOSTIC USE OF THE C-TERMINAL FRAGMENT rLb70(513–663). J Parasitol 1 April 2003; 89 (2): 372–378. doi: https://doi.org/10.1645/0022-3395(2003)089[0372:CACOTL]2.0.CO;2
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