The purpose of the present study was to use direct plasmid deoxyribonucleic acid (DNA) injection to identify specific antigens that confer protection against congenital transfer of Neospora caninum. Inbred BALB/c mice were vaccinated before pregnancy with a recombinant plasmid containing sequences encoding N. caninum antigen NcGRA7 or NcsHSP33. The mice were challenged with N. caninum tachyzoites at 10–12 days of gestation. Whereas 100% of pups born from dams immunized with control plasmid contained detectable levels of N. caninum DNA in a Neospora-specific polymerase chain reaction assay, only 46% of pups from pCMVi-NcGRA7–immunized mice and 53% of pCMVi-NcsHSP33–immunized mice were N. caninum positive, and none of the mice immunized with tachyzoite extract contained N. caninum DNA. Thus, immunization of mice with plasmid DNA expressing N. caninum antigens conferred partial protection against congenital neosporosis.
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June 2003
Research Article|
June 01 2003
IMMUNIZATION OF MICE WITH PLASMID DNA CODING FOR NcGRA7 OR NcsHSP33 CONFERS PARTIAL PROTECTION AGAINST VERTICAL TRANSMISSION OF NEOSPORA CANINUM
Susan Liddell;
Susan Liddell
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705. mjenkins@anri.barc.usda.gov
* Division of Agricultural Sciences, University of Nottingham, Leicestershire LE12 5RD, U.K
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Carolyn Parker;
Carolyn Parker
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705. mjenkins@anri.barc.usda.gov
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Bryan Vinyard;
Bryan Vinyard
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705. mjenkins@anri.barc.usda.gov
† Biometrics Consulting Unit, Beltsville Agricultural Research Center, Agricultural Research Service, United States Department of Agriculture, Building 005, Beltsville, Maryland 20705
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Mark Jenkins;
Mark Jenkins
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705. mjenkins@anri.barc.usda.gov
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J. P. Dubey
J. P. Dubey
Parasite Biology, Epidemiology and Systematics Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705. mjenkins@anri.barc.usda.gov
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J Parasitol (2003) 89 (3): 496–500.
Citation
Susan Liddell, Carolyn Parker, Bryan Vinyard, Mark Jenkins, J. P. Dubey; IMMUNIZATION OF MICE WITH PLASMID DNA CODING FOR NcGRA7 OR NcsHSP33 CONFERS PARTIAL PROTECTION AGAINST VERTICAL TRANSMISSION OF NEOSPORA CANINUM. J Parasitol 1 June 2003; 89 (3): 496–500. doi: https://doi.org/10.1645/GE-2969
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