This study compared specific polymerase chain reaction (PCR) primers and phylogenetic tree analysis of restriction fragment length polymorphism using the small subunit ribosomal RNA gene in various Blastocystis populations. A phylogenetic tree was constructed using 12 restriction enzymes and a sample pool of 22 isolates, including 2 reference strains and Proteromonas lacertae as an outgroup. The analysis showed that the 22 isolates could be separated into 7 clusters. Four of the 7 clusters were mixed groups that comprised isolates from both humans and nonhuman hosts. The other 3 clusters contained isolates from humans or nonhuman hosts only. The phylogenetic analysis also showed that B. hominis isolates from geographical separated areas did not necessarily cluster in the genetically different groups. The results of genetic homology and phylogenetic tree analysis among Blastocystis isolates from humans and animals indicated that all isolates from animals appear to be B. hominis. Polymerase chain reaction amplifications using previously described and newly defined specific primers mirrored the clusters obtained by the phylogenetic tree analysis. Our results show that primer PCR can be used as a powerful tool for the typing of Blastocystis populations.
Skip Nav Destination
Article navigation
June 2003
SYSTEMATICS-PHYLOGENETICS|
June 01 2003
MOLECULAR COMPARATIVE STUDIES AMONG BLASTOCYSTIS ISOLATES OBTAINED FROM HUMANS AND ANIMALS
Hisao Yoshikawa;
Hisao Yoshikawa
Department of Biological Science, Faculty of Science, Nara Women's University, Kitauoya-Nishimachi, Nara 630-8506, Japan. sb56013@cc.nara-wu.ac.jp
Search for other works by this author on:
Zhiliang Wu;
Zhiliang Wu
Department of Biological Science, Faculty of Science, Nara Women's University, Kitauoya-Nishimachi, Nara 630-8506, Japan. sb56013@cc.nara-wu.ac.jp
* Department of Parasitology, School of Medicine, Gifu University, Tsukasa, Gifu 500-8705, Japan
Search for other works by this author on:
Isao Nagano;
Isao Nagano
Department of Biological Science, Faculty of Science, Nara Women's University, Kitauoya-Nishimachi, Nara 630-8506, Japan. sb56013@cc.nara-wu.ac.jp
* Department of Parasitology, School of Medicine, Gifu University, Tsukasa, Gifu 500-8705, Japan
Search for other works by this author on:
Yuzo Takahashi
Yuzo Takahashi
Department of Biological Science, Faculty of Science, Nara Women's University, Kitauoya-Nishimachi, Nara 630-8506, Japan. sb56013@cc.nara-wu.ac.jp
* Department of Parasitology, School of Medicine, Gifu University, Tsukasa, Gifu 500-8705, Japan
Search for other works by this author on:
J Parasitol (2003) 89 (3): 585–594.
Citation
Hisao Yoshikawa, Zhiliang Wu, Isao Nagano, Yuzo Takahashi; MOLECULAR COMPARATIVE STUDIES AMONG BLASTOCYSTIS ISOLATES OBTAINED FROM HUMANS AND ANIMALS. J Parasitol 1 June 2003; 89 (3): 585–594. doi: https://doi.org/10.1645/0022-3395(2003)089[0585:MCSABI]2.0.CO;2
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
EVALUATION OF PELIBUEY LAMBS BORN TO MOTHERS PHENOTYPICALLY SEGREGATED ACCORDING TO RESISTANCE TO GASTROINTESTINAL NEMATODES IN THE HUMID TROPICS OF MEXICO
Claudia Virginia Zaragoza-Vera, Roberto Gonzalez-Garduño, Maritza Zaragoza-Vera, Guadalupe Arjona-Jimenez, Antonio Ortega-Pacheco, Oswaldo Margarito Torres-Chable
ACCEPTANCE OF THE 2022 CLARK P. READ MENTOR AWARD: MENTORING BY DESIGN
Richard E. Clopton