A soluble antigen isolated from Eimeria stiedai merozoites with a molecular mass of 49 kDa was detected in the bile of infected rabbits. Rabbits immunized with the antigen shed a lower number of oocysts than did nonimmunized rabbits postchallenge (p.c.). The immunized rabbits showed a marked and transient increase of alanine-aminotransferase (ALT) activity on day 8 p.c. The blood indocyanine green (ICG) clearance and r-glutamyltransferase (GGT) activity showed no change throughout the experiment. However, nonimmunized rabbits showed a gradual increase of ALT and GGT in the plasma and a delay of ICG p.c. Many merozoites were observed in the biliary ducts of the nonimmunized rabbits on day 8 p.c. using standard histology. In contrast, in the immunized rabbits, many inflammatory cells were observed around the biliary ducts, but there were few parasites in the tissue. These results suggest that the 49-kDa soluble protein antigen detected in the bile of the infected rabbits was a merozoite-specific antigen, and the immune reaction to the antigen may induce protective effects against the infection.
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June 2003
RESEARCH NOTES|
June 01 2003
Eimeria stiedai Merozoite 49-kDa Soluble Antigen Induces Protection Against Infection Available to Purchase
S. Hanada;
S. Hanada
aDepartment of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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Y. Umemoto;
Y. Umemoto
aDepartment of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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Y. Omata;
Y. Omata
bTo whom correspondence should be addressed.
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T. Koyama;
T. Koyama
aDepartment of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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K. Nishiyama;
K. Nishiyama
aDepartment of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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Y. Kobayashi;
Y. Kobayashi
cDepartment of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan. To whom correspondence should be addressed. [email protected]
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H. Furuoka;
H. Furuoka
cDepartment of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan. To whom correspondence should be addressed. [email protected]
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T. Matsui;
T. Matsui
cDepartment of Veterinary Pathology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan. To whom correspondence should be addressed. [email protected]
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R. Maeda;
R. Maeda
aDepartment of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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A. Saito
A. Saito
aDepartment of Veterinary Physiology, Obihiro University of Agriculture and Veterinary Medicine, Obihiro 080-8555, Japan
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J Parasitol (2003) 89 (3): 613–617.
Citation
S. Hanada, Y. Umemoto, Y. Omata, T. Koyama, K. Nishiyama, Y. Kobayashi, H. Furuoka, T. Matsui, R. Maeda, A. Saito; Eimeria stiedai Merozoite 49-kDa Soluble Antigen Induces Protection Against Infection. J Parasitol 1 June 2003; 89 (3): 613–617. doi: https://doi.org/10.1645/0022-3395(2003)089[0613:ESMKSA]2.0.CO;2
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