Several potential cues for increased surface antigenic expression of lung-stage schistosomula, such as lack of glucose and amino acids and extremes of pH or HCO3− concentration, failed to alter the negligible larval reactivity with control, infection, or irradiated cercariae-vaccine serum in indirect membrane immunofluorescence. In contrast, incubation of larvae in 90% corn oil for 6 hr led to surface membrane changes, which allowed specific and strong binding of antibody from antischistosome sera. The data together indicated that the lung-stage worms' confinement of antigenic molecules in lipid-rich sites of the outer membrane could be reversed in vitro after exposure to corn oil, in a concentration- and time-dependent manner.
Incubation of Schistosoma mansoni Lung-Stage Schistosomula in Corn Oil Exposes Their Surface Membrane Antigenic Specificities
- Views Icon Views
- Share Icon Share
- Search Site
Rashika El Ridi, Shadia H. Mohamed, Hatem Tallima; Incubation of Schistosoma mansoni Lung-Stage Schistosomula in Corn Oil Exposes Their Surface Membrane Antigenic Specificities. J Parasitol 1 October 2003; 89 (5): 1064–1067. doi: https://doi.org/10.1645/GE-3122RN
Download citation file: