Perkinsus marinus is responsible for disease and mortality of the American oyster, Crassostrea virginica. To investigate the interactions between P. marinus and oyster hemocytes, protease activity was measured in plasma of oysters collected 4 hr, 24 hr, 4 days, and 2 mo after experimental infection with P. marinus. A significant increase in protease activity was observed in oyster plasma 4 hr after injection with P. marinus, followed by a sharp decrease within 24 hr. Gelatin-impregnated gel electrophoresis showed the presence of 2 major bands (60 and 112 kDa) and 3 less prevalent bands (35, 92, and 200 kDa) with metalloproteinaselike activity in the plasma of noninfected oysters. Additional bands in the 40- to 60-kDa range, corresponding to P. marinus serine proteases, were observed in oyster plasma at early time points after infection. A transient, but significant, decrease in the activity of oyster metalloproteinases was observed at early time points after infection. Coincubation of oyster plasma with P. marinus extracellular products resulted in a decrease in oyster metalloproteinases and several P. marinus proteases. This study provides insights into the role of proteases in the pathogenesis of Dermo disease.
Skip Nav Destination
Article navigation
October 2003
INVERTEBRATE-PARASITE RELATIONSHIPS|
October 01 2003
PROTEASE ACTIVITY IN THE PLASMA OF AMERICAN OYSTERS, CRASSOSTREA VIRGINICA, EXPERIMENTALLY INFECTED WITH THE PROTOZOAN PARASITE PERKINSUS MARINUS
P. Muñoz;
P. Muñoz
Department of Fisheries, Animal, and Veterinary Science, University of Rhode Island, 23 Woodward Hall, Kingston, Rhode Island 02881. [email protected]
* Present address: Departamento de Biología Celular, Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain
Search for other works by this author on:
K. Vance;
K. Vance
Department of Fisheries, Animal, and Veterinary Science, University of Rhode Island, 23 Woodward Hall, Kingston, Rhode Island 02881. [email protected]
Search for other works by this author on:
M. Gómez-Chiarri
M. Gómez-Chiarri
Department of Fisheries, Animal, and Veterinary Science, University of Rhode Island, 23 Woodward Hall, Kingston, Rhode Island 02881. [email protected]
† To whom correspondence should be addressed
Search for other works by this author on:
J Parasitol (2003) 89 (5): 941–951.
Citation
P. Muñoz, K. Vance, M. Gómez-Chiarri; PROTEASE ACTIVITY IN THE PLASMA OF AMERICAN OYSTERS, CRASSOSTREA VIRGINICA, EXPERIMENTALLY INFECTED WITH THE PROTOZOAN PARASITE PERKINSUS MARINUS. J Parasitol 1 October 2003; 89 (5): 941–951. doi: https://doi.org/10.1645/GE-3126
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
CLITELLATE (ANNELIDA: CLITELLATA) PARASITES AND PREDATORS OF NORTH AMERICAN HERPETOFAUNA: CHECKLIST OF SPECIES, IDENTIFICATION KEY, AND A NEW RECORD FOR MEXICO
Manuel De Luna, Roberto García-Barrios, Diane P. Barton, Leonardo García-Vázquez
LOUSY ASSOCIATIONS: SUCKING LICE (PHTHIRAPTERA: ANOPLURA) PARASITIZING RODENTS AND LAGOMORPHS IN THE DESERT NATIONAL WILDLIFE REFUGE, NEVADA
Sara B. Weinstein, John P. Dumbacher, Lance A. Durden
A NEW SPECIES OF ACANTHOBOTHRIUM (CESTODA: ONCHOPROTEOCEPHALIDEA), PARASITE OF THE GIANT ELECTRIC RAY NARCINE ENTEMEDOR JORDAN AND STARKS, 1895 (BATOIDEA: TORPEDINIFORMES) FROM MEXICO
Erick Rodríguez-Ibarra, Berenice Adán-Torres, Fernando Ruiz-Escobar, Gerardo Torres-Carrera
SEROPREVALENCE OF TOXOPLASMA GONDII IN GOATS FROM SOUTHWESTERN MISSISSIPPI, USA
Alexander D. W. Acholonu, Jamela S. Alexander
TAXES OF DICYEMIDS (PHYLUM DICYEMIDA)
Naoki Hisayama, Yuto Takeuchi, Hidetaka Furuya