Perkinsus marinus is responsible for disease and mortality of the American oyster, Crassostrea virginica. To investigate the interactions between P. marinus and oyster hemocytes, protease activity was measured in plasma of oysters collected 4 hr, 24 hr, 4 days, and 2 mo after experimental infection with P. marinus. A significant increase in protease activity was observed in oyster plasma 4 hr after injection with P. marinus, followed by a sharp decrease within 24 hr. Gelatin-impregnated gel electrophoresis showed the presence of 2 major bands (60 and 112 kDa) and 3 less prevalent bands (35, 92, and 200 kDa) with metalloproteinaselike activity in the plasma of noninfected oysters. Additional bands in the 40- to 60-kDa range, corresponding to P. marinus serine proteases, were observed in oyster plasma at early time points after infection. A transient, but significant, decrease in the activity of oyster metalloproteinases was observed at early time points after infection. Coincubation of oyster plasma with P. marinus extracellular products resulted in a decrease in oyster metalloproteinases and several P. marinus proteases. This study provides insights into the role of proteases in the pathogenesis of Dermo disease.
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October 2003
Research Article|
October 01 2003
PROTEASE ACTIVITY IN THE PLASMA OF AMERICAN OYSTERS, CRASSOSTREA VIRGINICA, EXPERIMENTALLY INFECTED WITH THE PROTOZOAN PARASITE PERKINSUS MARINUS
P. Muñoz;
P. Muñoz
Department of Fisheries, Animal, and Veterinary Science, University of Rhode Island, 23 Woodward Hall, Kingston, Rhode Island 02881. gomezchi@uri.edu
* Present address: Departamento de Biología Celular, Facultad de Biología, Universidad de Murcia, 30100 Murcia, Spain
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K. Vance;
K. Vance
Department of Fisheries, Animal, and Veterinary Science, University of Rhode Island, 23 Woodward Hall, Kingston, Rhode Island 02881. gomezchi@uri.edu
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M. Gómez-Chiarri
M. Gómez-Chiarri
Department of Fisheries, Animal, and Veterinary Science, University of Rhode Island, 23 Woodward Hall, Kingston, Rhode Island 02881. gomezchi@uri.edu
† To whom correspondence should be addressed
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J Parasitol (2003) 89 (5): 941–951.
Citation
P. Muñoz, K. Vance, M. Gómez-Chiarri; PROTEASE ACTIVITY IN THE PLASMA OF AMERICAN OYSTERS, CRASSOSTREA VIRGINICA, EXPERIMENTALLY INFECTED WITH THE PROTOZOAN PARASITE PERKINSUS MARINUS. J Parasitol 1 October 2003; 89 (5): 941–951. doi: https://doi.org/10.1645/GE-3126
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