The molecular mechanisms by which the malarial parasite has managed to develop resistance to many antimalarial drugs remain to be completely elucidated. Mutations in the pfmdr1 gene of Plasmodium falciparum, as well as an increase in pfmdr1 copy number, have been associated with resistance to the quinoline-containing antimalarial drugs. We investigated the mechanisms of drug resistance in Plasmodium using a collection of P. yoelii lines with different drug resistance profiles. The mdr1 gene of P. yoelii (pymdr1) was identified and characterized. A 2- to 3-fold increase in the pymdr1 gene copy number was observed in the P. yoelii ART line (artemisinin resistant) when compared with the NS parental line. The pymdr1 gene was mapped to a chromosome of 2.1 Mb in all lines analyzed. Reverse transcriptase–polymerase chain reaction and Western blot experiments confirmed the expression of the gene at the RNA and protein levels.
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February 2004
Research Article|
February 01 2004
PLASMODIUM YOELII: IDENTIFICATION AND PARTIAL CHARACTERIZATION OF AN MDR1 GENE IN AN ARTEMISININ-RESISTANT LINE
Iván Ferrer-Rodríguez;
Iván Ferrer-Rodríguez
Department of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, P.O. Box 365067, San Juan, Puerto Rico 00936-5067. aserrano@rcm.upr.edu
* Present address: Department of Natural Sciences and Mathematics, Inter American University, Bayamón Campus, Road John Will Harris, No. 500, Bayamón Puerto Rico 00957
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José Pérez-Rosado;
José Pérez-Rosado
Department of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, P.O. Box 365067, San Juan, Puerto Rico 00936-5067. aserrano@rcm.upr.edu
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Gary W. Gervais;
Gary W. Gervais
Department of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, P.O. Box 365067, San Juan, Puerto Rico 00936-5067. aserrano@rcm.upr.edu
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Wallace Peters;
Wallace Peters
Department of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, P.O. Box 365067, San Juan, Puerto Rico 00936-5067. aserrano@rcm.upr.edu
† Northwick Park Institute for Medical Research, Centre for Tropical Antiprotozoal Chemotherapy, Watford Road, Harrow, Middlesex HA1 3UJ, United Kingdom
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Brian L. Robinson;
Brian L. Robinson
Department of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, P.O. Box 365067, San Juan, Puerto Rico 00936-5067. aserrano@rcm.upr.edu
† Northwick Park Institute for Medical Research, Centre for Tropical Antiprotozoal Chemotherapy, Watford Road, Harrow, Middlesex HA1 3UJ, United Kingdom
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Adelfa E. Serrano
Adelfa E. Serrano
Department of Microbiology and Medical Zoology, University of Puerto Rico, School of Medicine, P.O. Box 365067, San Juan, Puerto Rico 00936-5067. aserrano@rcm.upr.edu
‡ To whom correspondence should be addressed
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J Parasitol (2004) 90 (1): 152–160.
Citation
Iván Ferrer-Rodríguez, José Pérez-Rosado, Gary W. Gervais, Wallace Peters, Brian L. Robinson, Adelfa E. Serrano; PLASMODIUM YOELII: IDENTIFICATION AND PARTIAL CHARACTERIZATION OF AN MDR1 GENE IN AN ARTEMISININ-RESISTANT LINE. J Parasitol 1 February 2004; 90 (1): 152–160. doi: https://doi.org/10.1645/GE-3225
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