Recently, several polymerase chain reaction (PCR)–based methods for detection and genetic identification of haemosporidian parasites in avian blood have been developed. Most of these have considerably higher sensitivity compared with traditional microscope-based examinations of blood smears. These new methods have already had a strong impact on several aspects of research on avian blood parasites. In this study, we present a new nested PCR approach, building on a previously published PCR method, which has significantly improved performance. We compare the new method with some existing assays and show, by sequence-based data, that the higher detection rate is mainly due to superior detection of Plasmodium spp. infections, which often are of low intensity and, therefore, hard to detect with other methods.
Skip Nav Destination
Article navigation
February 2004
RESEARCH NOTES|
February 01 2004
A New Nested Polymerase Chain Reaction Method Very Efficient in Detecting Plasmodium and Haemoproteus Infections From Avian Blood
J. Waldenström;
J. Waldenström
aDepartment of Animal Ecology, Ecology Building, Lund University, SE-223 62 Lund, Sweden
Search for other works by this author on:
S. Bensch;
S. Bensch
aDepartment of Animal Ecology, Ecology Building, Lund University, SE-223 62 Lund, Sweden
Search for other works by this author on:
D. Hasselquist;
D. Hasselquist
aDepartment of Animal Ecology, Ecology Building, Lund University, SE-223 62 Lund, Sweden
Search for other works by this author on:
Ö Östman
Ö Östman
bDepartment of Ecology and Crop Production Science, Swedish University of Agricultural Sciences, P.O. Box 7043, SE-750 07 Uppsala, Sweden. jonas.waldenstrom@zooekol.lu.se
Search for other works by this author on:
J Parasitol (2004) 90 (1): 191–194.
Citation
J. Waldenström, S. Bensch, D. Hasselquist, Ö Östman; A New Nested Polymerase Chain Reaction Method Very Efficient in Detecting Plasmodium and Haemoproteus Infections From Avian Blood. J Parasitol 1 February 2004; 90 (1): 191–194. doi: https://doi.org/10.1645/GE-3221RN
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
POPULATION STRUCTURE AND BARTONELLA QUINTANA IN HEAD AND BODY LICE IN POKHARA, NEPAL (ANOPLURA: PEDICULIDAE)
Shreekanta S. Poudel, Jefferson A. Vaughan
PALEOEPIDEMIOLOGY OF DIPHYLLOBOTHRIOSIS: CULTURAL AND ENVIRONMENTAL FACTORS AFFECTING ADENOCEPHALUS INTENSITY AND PREVALENCE
Karl J. Reinhard, Bernardo Arriaza, William Alexander Avery, Jane Buikstra, Morgana Camacho, Elizabeth Goodman, John Obafunwa, Bruce Owen, Isabel Teixeira-Santos
TAXONOMIC REASSESSMENT AND MORPHOLOGICAL REDESCRIPTION OF NEMATOSTRIGEA SERPENS ANNULATA (DIGENEA: STRIGEIDAE) FROM OSPREY IN NORTH AMERICA
Bharani Gudla, Sarah A. Orlofske, Sara V. Brant, Vasyl V. Tkach, Shelli Dubay, Lauren Holtz, Tyler J. Achatz
PRESCRIBING PATTERNS AND THE USE OF ANTHELMINTIC DRUGS IN COLOMBIAN PATIENTS: A CROSS-SECTIONAL STUDY
Maria Camila Montes-Montoya, Andrés Gaviria-Mendoza, Maria Mónica Murillo-Muñoz, Jaime Andrés Cardona-Ospina, Jorge Enrique Machado-Alba
MOLECULAR CHARACTERIZATION OF ORNITHOSTRONGYLUS QUADRIRADIATUS ISOLATED FROM A RACING PIGEON (COLUMBA LIVIA DOMESTICA)
Aleksandra Ledwoń, Ines Szotowska, Izabella Dolka, Piotr Szeleszczuk