In the present study, we compared the effect of 5% oxygen tension (hypoxia) with a normal tension of 21% oxygen (normoxia) on macrophage infection by the protozoan parasite Leishmania amazonensis. Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal macrophages) exposed to hypoxia showed a reduction of the percentage of infected cells and the number of intracellular parasites per cell. Observations on the kinetics of infection indicated that hypoxia did not depress L. amazonensis phagocytosis but induced macrophages to reduce intracellular parasitism. Furthermore, hypoxia did not act synergistically with γ-interferon and bacterial lipopolysaccharides in macrophages to induce killing of parasites. Experiments also indicated no correlation between nitric oxide production and control of infection in macrophages under hypoxic condition. Thus, we have provided the first evidence that hypoxia, which occurs in various pathological conditions, can alter macrophage susceptibility to a parasitic infection.
Skip Nav Destination
Article navigation
June 2004
Research Article|
June 01 2004
EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS
Marcelle Carolina Colhone;
Marcelle Carolina Colhone
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
Search for other works by this author on:
Wagner Welber Arrais-Silva;
Wagner Welber Arrais-Silva
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
Search for other works by this author on:
Claudia Picoli;
Claudia Picoli
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
Search for other works by this author on:
Selma Giorgio
Selma Giorgio
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
* To whom correspondence should be addressed
Search for other works by this author on:
J Parasitol (2004) 90 (3): 510–515.
Citation
Marcelle Carolina Colhone, Wagner Welber Arrais-Silva, Claudia Picoli, Selma Giorgio; EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS. J Parasitol 1 June 2004; 90 (3): 510–515. doi: https://doi.org/10.1645/GE-3286
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
CERCARIAL LONGEVITY AND INFECTIVITY OF BOLBOPHORUS DAMNIFICUS, WITH NOTES ON METACERCARIAL PERSISTENCE AND SITE SPECIFICITY IN CHANNEL AND HYBRID CATFISH
Mackenzie A. Gunn, Peter J. Allen, T. Graham Rosser, David J. Wise, Matt J. Griffin
DETECTION OF SPLENDIDOFILARIA SP. (ONCHOCERCIDAE:SPLENDIDOFILARIINAE) MICROFILARIA WITHIN ALASKAN GROUND-DWELLING BIRDS IN THE GROUSE SUBFAMILY TETRAONINAE USING TAQMAN PROBE-BASED REAL-TIME PCR
Stephen E. Greiman, Robert E. Wilson, Briana Sesmundo, Jack Reakoff, Sarah A. Sonsthagen
OCCURRENCE OF EUSTRONGYLIDES EXCISUS (NEMATODA:DIOCTOPHYMATIDAE) IN EUROPEAN PERCH (PERCA FLUVIATILIS) AND GREAT CORMORANT (PHALACROCORAX CARBO) IN LAKE ANNONE, NORTHERN ITALY
Aurora Rusconi, Paola Prati, Roldano Bragoni, Michele Castelli, Umberto Postiglione, Sara Rigamonti, Davide Sassera, Emanuela Olivieri
REVIEW OF THE ROLE OF PARASITIC NEMATODE EXCRETORY/SECRETORY PROTEINS IN HOST IMMUNOMODULATION
Ogadinma K. Okakpu, Adler R. Dillman
REVIEW OF DIOCTOPHYME RENALE: ETIOLOGY, MORPHOLOGY, BIOLOGY, ECOEPIDEMIOLOGY, PATHOGENESIS, SYMPTOMATOLOGY, DIAGNOSIS, TREATMENT, AND PREVENTION
Zully Hernández Russo, Jorge Cárdenas Callirgos, Alberto García-Ayachi, Eric J. Wetzel