In the present study, we compared the effect of 5% oxygen tension (hypoxia) with a normal tension of 21% oxygen (normoxia) on macrophage infection by the protozoan parasite Leishmania amazonensis. Macrophages from different sources (human cell line U937, murine cell line J774, and murine peritoneal macrophages) exposed to hypoxia showed a reduction of the percentage of infected cells and the number of intracellular parasites per cell. Observations on the kinetics of infection indicated that hypoxia did not depress L. amazonensis phagocytosis but induced macrophages to reduce intracellular parasitism. Furthermore, hypoxia did not act synergistically with γ-interferon and bacterial lipopolysaccharides in macrophages to induce killing of parasites. Experiments also indicated no correlation between nitric oxide production and control of infection in macrophages under hypoxic condition. Thus, we have provided the first evidence that hypoxia, which occurs in various pathological conditions, can alter macrophage susceptibility to a parasitic infection.
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June 2004
IMMUNOLOGY|
June 01 2004
EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS
Marcelle Carolina Colhone;
Marcelle Carolina Colhone
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
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Wagner Welber Arrais-Silva;
Wagner Welber Arrais-Silva
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
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Claudia Picoli;
Claudia Picoli
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
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Selma Giorgio
Selma Giorgio
Departamento de Parasitologia, Instituto de Biologia, Universidade Estadual de Campinas, CxP 6109, 13087-970 Campinas, SP, Brazil. sgiorgio@unicamp.br
* To whom correspondence should be addressed
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J Parasitol (2004) 90 (3): 510–515.
Citation
Marcelle Carolina Colhone, Wagner Welber Arrais-Silva, Claudia Picoli, Selma Giorgio; EFFECT OF HYPOXIA ON MACROPHAGE INFECTION BY LEISHMANIA AMAZONENSIS. J Parasitol 1 June 2004; 90 (3): 510–515. doi: https://doi.org/10.1645/GE-3286
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