Here, we analyzed histological findings and parasite burden in chronic Neospora caninum infection in BALB/c and ICR mice and studied the correlation between lesion severity and parasite load in brain. To obtain a better understanding of the infection, we examined the influence of various host–pathogen factors. Groups of outbred (ICR) and inbred (BALB/c) mice were inoculated using several NC-1 parasite doses (4 × 105, 106, and 5 × 106 tachyzoites), inoculation routes (intraperitoneal and subcutaneous), and 3 immunosuppressive treatments (methylprednisolone, cyclophosphamide, and vinblastine). Lesion severity was analyzed in the liver, lung, heart, and brain tissues, and parasite load was measured by real-time polymerase chain reaction in brain tissue. The results indicated more severe cerebral lesions and higher brain parasite burdens in inbred than in outbred mice. Hepatic tissue was the primary lesion site in immunosuppressed ICR mice. We also observed that increased inoculum size was reflected in greater lesion severity and a higher cerebral parasite load. No difference was observed with respect to inoculation route. The study also showed an association between brain parasite burden and severity of cerebral lesions in BALB/c mice.
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June 2004
PATHOLOGY|
June 01 2004
CHARACTERIZATION OF PATHOLOGY AND PARASITE LOAD IN OUTBRED AND INBRED MOUSE MODELS OF CHRONIC NEOSPORA CANINUM INFECTION
Esther Collantes-Fernández;
Esther Collantes-Fernández
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain. [email protected]
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Gema Álvarez-García;
Gema Álvarez-García
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain. [email protected]
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Valentín Pérez-Pérez;
Valentín Pérez-Pérez
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain. [email protected]
* Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Spain
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Juana Pereira-Bueno;
Juana Pereira-Bueno
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain. [email protected]
* Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de León, Spain
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Luis Miguel Ortega-Mora
Luis Miguel Ortega-Mora
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, 28040 Madrid, Spain. [email protected]
† To whom correspondence should be addressed
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J Parasitol (2004) 90 (3): 579–583.
Citation
Esther Collantes-Fernández, Gema Álvarez-García, Valentín Pérez-Pérez, Juana Pereira-Bueno, Luis Miguel Ortega-Mora; CHARACTERIZATION OF PATHOLOGY AND PARASITE LOAD IN OUTBRED AND INBRED MOUSE MODELS OF CHRONIC NEOSPORA CANINUM INFECTION. J Parasitol 1 June 2004; 90 (3): 579–583. doi: https://doi.org/10.1645/GE-3290
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