The purpose of the present study was to characterize the intracellular distribution of a native Neospora caninum 56-kDa protein that is recognized by sera from N. caninum–infected dairy cattle. The complementary DNA coding for this protein was expressed in Escherichia coli as a polyHis fusion protein to which antiserum was prepared and used to localize the antigen in N. caninum tachyzoites and bradyzoites. By sodium dodecylsulfate–polyacrylamide gel electrophoresis and immunoblotting, antirecombinant Nc56 serum recognized a major 56-kDa protein and 2 minor (43 and 39 kDa) proteins of N. caninum tachyzoites. Antiserum to recombinant 56-kDa protein showed this antigen to be present in both N. caninum tachyzoites and bradyzoites/cysts as detected by immunofluorescence staining. Immunoelectron microscopy revealed the 56-kDa antigen to be present in the apical end of both tachyzoites and bradyzoites and possibly extracellularly secreted by tachyzoites.
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June 2004
RESEARCH NOTES|
June 01 2004
Localization of a 56-kDa Antigen That is Present in Multiple Developmental Stages of Neospora caninum
Mark Jenkins;
Mark Jenkins
aAnimal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
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Rodrigo Soares;
Rodrigo Soares
aAnimal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
bDepartmento de Medicina Veterinaria Preventiva e Saude Animal, Universidade de São Paulo, São Paulo, SP, Brazil
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Charles Murphy;
Charles Murphy
aAnimal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
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Andrew Hemphill;
Andrew Hemphill
aAnimal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
cInstitute of Parasitology, University of Berne, Langgass-Strasse 122, Berne, CH-3012, Switzerland
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Ryan O'Handley;
Ryan O'Handley
aAnimal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
dAtlantic Veterinary College, University of Prince Edward Island, Charlottetown, PEI, C1A 4P3, Canada. [email protected]
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J. P. Dubey
J. P. Dubey
aAnimal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
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J Parasitol (2004) 90 (3): 660–663.
Citation
Mark Jenkins, Rodrigo Soares, Charles Murphy, Andrew Hemphill, Ryan O'Handley, J. P. Dubey; Localization of a 56-kDa Antigen That is Present in Multiple Developmental Stages of Neospora caninum. J Parasitol 1 June 2004; 90 (3): 660–663. doi: https://doi.org/10.1645/GE-3296RN
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