In the present study, monoclonal antibodies (mAbs) against adult Ornithodoros moubata hemocytes were established. Afterward, artificial feeding was performed to assess the tickcidal effect of fetal bovine serum meal containing each mAb. As a result, Om21 showed the strongest tickcidal effect on adult female O. moubata. The reactivity of various tick cells and organs, including the hemocyte, midgut, trachea, ovary, fat body, and muscle, to Om21 was then examined by an indirect immunofluorescent antibody test and by immunoelectron microscopy. Om21 reacted with not only hemocytes but also with fat body cells, epidermis, cuticle of the trachea, connective tissue of the muscle, and the basement membrane of the midgut, trachea, fat body, oocyte, and epidermis. These results suggest that Om21 passing through the midgut epithelium induced a tickcidal effect on hemocytes or various organs. However, the target of Om21 could not be identified in the present study. The antihemocyte mAb produced in this study, Om21, may be useful for the immunological control of ticks.
Skip Nav Destination
Article navigation
August 2004
ECTOPARASITOLOGY|
August 01 2004
TICKCIDAL EFFECT OF MONOCLONAL ANTIBODIES AGAINST HEMOCYTES, OM21, IN AN ADULT FEMALE TICK, ORNITHODOROS MOUBATA (ACARI: ARGASIDAE)
Tomohide Matsuo;
Tomohide Matsuo
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. [email protected]
Search for other works by this author on:
Noboru Inoue;
Noboru Inoue
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. [email protected]
Search for other works by this author on:
Martin R. Ruheta;
Martin R. Ruheta
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. [email protected]
Search for other works by this author on:
DeMar Taylor;
DeMar Taylor
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. [email protected]
* Institute of Agriculture and Forestry, University of Tsukuba, Tennohdai, Tsukuba, Ibaraki 305-0003, Japan
Search for other works by this author on:
Kozo Fujisaki
Kozo Fujisaki
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan. [email protected]
† To whom correspondence should be addressed
Search for other works by this author on:
J Parasitol (2004) 90 (4): 715–720.
Citation
Tomohide Matsuo, Noboru Inoue, Martin R. Ruheta, DeMar Taylor, Kozo Fujisaki; TICKCIDAL EFFECT OF MONOCLONAL ANTIBODIES AGAINST HEMOCYTES, OM21, IN AN ADULT FEMALE TICK, ORNITHODOROS MOUBATA (ACARI: ARGASIDAE). J Parasitol 1 August 2004; 90 (4): 715–720. doi: https://doi.org/10.1645/GE-164R1
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
PREVALENCE OF DIROFILARIA MICROFILARIA IN AMERICAN BLACK BEARS (URSUS AMERICANUS) IN PENNSYLVANIA, 2018–2020
Alia Mazzatosta, Christopher A. Cleveland, Michael J. Yabsley, Ellen Haynes, Hannah S. Tiffin, Erika T. Machtinger, Joshua B. Johnson, Mark Ternent, Emily Carrollo, Justin D. Brown
A NEW MYXIDIUM SPECIES FROM THE LEAST KILLIFISH HETERANDRIA FORMOSA IN LOUISIANA
Christopher M. Whipps, Emily C. Ogburn, William F. Font
GENOMICS AND MORPHOLOGY RESOLVE CHIPMUNK SUCKING LOUSE SYSTEMATICS (GENUS HOPLOPLEURA)
Priscilla A. San Juan, Lance A. Durden, Julie M. Allen, Anna J. Phillips, Kayce C. Bell
MORPHOLOGICAL OBSERVATION AND DETAILED MOLECULAR CHARACTERIZATION OF FISCHOEDERIUS ELONGATUS (DIGENEA: GASTROTHYLACIDAE) FROM THE RUMEN OF DOMESTIC CATTLE IN CAMBODIA
Chinda Wann, Bengthay Tep, Witaya Suriyasathaporn, Yasuhiro Morita, Vutha Pheng, Satoshi Ohkura, Shuichi Matsuyama, Sho Nakamura, Kei Hayashi