This work reports the detection of specific immunoglobulins (Ig) against rFh8, a recombinant Fasciola hepatica adult worm excretion–secretion antigen, in sera from experimentally (rabbit, Wistar rat, cattle, and sheep), or naturally (human) infected hosts. In the case of laboratory experimental models the study revealed significant differences between rabbits, which recognized the recombinant antigen all along the infection, and Wistar rats, which showed high anti-rFh8 Ig levels only for a short period of the infection. Available sera from experimentally infected cattle and sheep, as well as sera from naturally F. hepatica–infected humans, also contained significant levels of Ig against rFh8, suggesting that Fh8 was produced by F. hepatica at a very early stage of infection in all hosts so far analyzed and that the rFh8 antigen could be used as a tool for the diagnosis of F. hepatica infections.
Skip Nav Destination
Article navigation
August 2004
IMMUNOLOGY|
August 01 2004
A RECOMBINANT ANTIGEN RECOGNIZED BY FASCIOLA HEPATICA–INFECTED HOSTS
Elisabete Silva;
Elisabete Silva
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
Search for other works by this author on:
Antonio Castro;
Antonio Castro
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
* To whom correspondence should be addressed
Search for other works by this author on:
António Lopes;
António Lopes
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
Search for other works by this author on:
Artur Rodrigues;
Artur Rodrigues
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
† Instituto de Biologia Molecular e Celular do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal
Search for other works by this author on:
César Dias;
César Dias
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
Search for other works by this author on:
Antónia Conceição;
Antónia Conceição
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
‡ Escola Superior Agrária de Coimbra, Bencanta, 3040-316, Coimbra, Portugal
Search for other works by this author on:
José Alonso;
José Alonso
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
§ Departamento de Bioquímica y Biología Molecular. Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain
Search for other works by this author on:
J. M. Correia da Costa;
J. M. Correia da Costa
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
∥ Centro de Estudos de Ciência Animal, ICETA-CECA, Rua D. Manuel II, Apartado 55142, 4051-401 Porto, Portugal
Search for other works by this author on:
Maria Bastos;
Maria Bastos
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
Search for other works by this author on:
Francisco Parra;
Francisco Parra
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
§ Departamento de Bioquímica y Biología Molecular. Instituto Universitario de Biotecnología de Asturias, Universidad de Oviedo, 33006 Oviedo, Spain
Search for other works by this author on:
Pedro Moradas-Ferreira;
Pedro Moradas-Ferreira
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
† Instituto de Biologia Molecular e Celular do Porto, Rua do Campo Alegre, 823, 4150-180, Porto, Portugal
Search for other works by this author on:
Maria Silva
Maria Silva
Laboratório de Parasitologia, Centro de Imunologia e Biologia Parasitária, Instituto Nacional de Saúde Dr. Ricardo Jorge do Porto, Rua de São Luís N° 16, 4000-509 Porto, Portugal. [email protected]
Search for other works by this author on:
J Parasitol (2004) 90 (4): 746–751.
Citation
Elisabete Silva, Antonio Castro, António Lopes, Artur Rodrigues, César Dias, Antónia Conceição, José Alonso, J. M. Correia da Costa, Maria Bastos, Francisco Parra, Pedro Moradas-Ferreira, Maria Silva; A RECOMBINANT ANTIGEN RECOGNIZED BY FASCIOLA HEPATICA–INFECTED HOSTS. J Parasitol 1 August 2004; 90 (4): 746–751. doi: https://doi.org/10.1645/GE-136R
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
LOUSY ASSOCIATIONS: SUCKING LICE (PHTHIRAPTERA: ANOPLURA) PARASITIZING RODENTS AND LAGOMORPHS IN THE DESERT NATIONAL WILDLIFE REFUGE, NEVADA
Sara B. Weinstein, John P. Dumbacher, Lance A. Durden
A NEW SPECIES OF ACANTHOBOTHRIUM (CESTODA: ONCHOPROTEOCEPHALIDEA), PARASITE OF THE GIANT ELECTRIC RAY NARCINE ENTEMEDOR JORDAN AND STARKS, 1895 (BATOIDEA: TORPEDINIFORMES) FROM MEXICO
Erick Rodríguez-Ibarra, Berenice Adán-Torres, Fernando Ruiz-Escobar, Gerardo Torres-Carrera
SEROPREVALENCE OF TOXOPLASMA GONDII IN GOATS FROM SOUTHWESTERN MISSISSIPPI, USA
Alexander D. W. Acholonu, Jamela S. Alexander
TAXES OF DICYEMIDS (PHYLUM DICYEMIDA)
Naoki Hisayama, Yuto Takeuchi, Hidetaka Furuya
TRICHINELLA MURRELLI POZIO AND LA ROSA, 2000 IN A GRAY FOX (UROCYON CINEREOARGENTEUS) FROM PENNSYLVANIA: A NEW HOST RECORD FOR THIS ZOONOTIC NEMATODE
P. C. Thompson, L. S. de Araujo, A. Gupta, S. Kay, O. C. H. Kwok, J. Battle, K. Van Why, J. D. Brown, B. M. Rosenthal, J. P. Dubey