The molecular mechanism of benzimidazole (BZ) resistance in cyathostomins of horses is still unclear. Previous studies revealed that the TTC or TAC polymorphism in codon 200 of the beta-tubulin isotype 1 gene is not as strictly correlated with BZ resistance as in trichostrongyles in sheep. To identify further sites of polymorphism within the beta-tubulin gene related to BZ resistance, complete complementary DNAs (cDNAs) encoding beta-tubulin of adult worms of Cylicocyclus nassatus, Cyathostomum pateratum, Cyathostomum coronatum, Cyathostomum catinatum, Cylicostephanus longibursatus, and Cylicostephanus goldi of a BZ-resistant cyathostomin population were characterized using specific primers. The cDNA sequence of each species spans 1,429 bp, encoding a protein of 448 amino acids. The interspecific identities are 95.2–99.6% at the nucleotide and 98.7–100.0% at the peptide level. The comparison of the amino acid sequences of individuals isolated from the BZ-resistant cyathostomin population with those from individuals of Cc. nassatus, Cy. coronatum, Cy. pateratum, and Cy. catinatum of a BZ-susceptible one showed differing amino acids in 11 positions. The commonness of a phenylalanine to tyrosine mutation at position 167 in all the 6 cyathostomin species isolated from a BZ-resistant population suggests its involvement in the molecular mechanism in BZ resistance.
Skip Nav Destination
Article navigation
August 2004
RESEARCH NOTES|
August 01 2004
Beta-Tubulin Complementary DNA Sequence Variations Observed Between Cyathostomins From Benzimidazole-Susceptible and -Resistant Populations
M. Drogemuller;
M. Drogemuller
Institute of Parasitology, Hannover School of Veterinary Medicine, Buenteweg 17, Hannover D-30559, Germany. [email protected]
Search for other works by this author on:
T. Schnieder;
T. Schnieder
Institute of Parasitology, Hannover School of Veterinary Medicine, Buenteweg 17, Hannover D-30559, Germany. [email protected]
Search for other works by this author on:
Gvon Samson-Himmelstjerna
Gvon Samson-Himmelstjerna
Institute of Parasitology, Hannover School of Veterinary Medicine, Buenteweg 17, Hannover D-30559, Germany. [email protected]
Search for other works by this author on:
J Parasitol (2004) 90 (4): 868–870.
Citation
M. Drogemuller, T. Schnieder, Gvon Samson-Himmelstjerna; Beta-Tubulin Complementary DNA Sequence Variations Observed Between Cyathostomins From Benzimidazole-Susceptible and -Resistant Populations. J Parasitol 1 August 2004; 90 (4): 868–870. doi: https://doi.org/10.1645/GE3305RN
Download citation file:
Sign in
Don't already have an account? Register
Client Account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Sign in via your Institution
Sign in via your InstitutionCiting articles via
SPECIES OF HEMIRHAMPHICULUS (MONOGENOIDEA: DACTYLOYRIDAE) INFECTING THE GILL LAMELLAE OF SOME BELONIFORM FISHES IN WATERS OF THE GULF OF MEXICO OFF FLORIDA, WITH DESCRIPTIONS OF FIVE NEW SPECIES AND COMMENTS ON THE DACTYLOGYRID GENERA HAVING SPECIES INFECTING BELONIFORM FISHES
Delane C. Kritsky, Micah D. Bakenhaster
HEPATOZOON RUFI N. SP. (APICOMPLEXA: HEPATOZOIDAE) OF BOBCATS (LYNX RUFUS) FROM MISSISSIPPI
J. P. Dubey, Aditya Gupta, Larissa S. de Araujo, Oliver C. H. Kwok, B. M. Rosenthal
LIFE CYCLE VARIATION AND HOST–PARASITE INTERACTIONS OF FOUR CONGENERIC SPECIES OF TURTLE ACANTHOCEPHALANS
Ryan W. Koch, Yetkin Ipek, Matthew G. Bolek
PHYLOGENY AND SYSTEMATICS OF CYATHOCOTYLID DIGENEANS (DIGENEA: DIPLOSTOMOIDEA) PARASITIZING SNAKES WITH DESCRIPTION OF THREE NEW SPECIES OF GOGATEA FROM AUSTRALIA AND VIETNAM
Tyler J. Achatz, Zoe S. Von Holten, Tran Thi Binh, Vasyl V. Tkach