This study was undertaken to observe the effects of hydrogen peroxide on Cryptosporidium parvum oocysts with respect to protease activity in comparison to known protease inhibitors. In assessing the possible mechanisms of action of hydrogen peroxide, treatment effectiveness was analyzed using 3 assays and the potential roles of proteases and cations were considered. Treatment of C. parvum oocysts with hydrogen peroxide inhibited protease activity up to 50% compared with untreated controls. Treatment of oocysts with chemicals that affect sulfhydryls, including N-ethylmaleimide and dithiolthreitol, inhibited protease activity by >90%. Treatment of oocysts with these chemicals, along with the protease inhibitors, phenylmethylsulfonyl fluoride (PMSF), ethylenediamine-tetraacetic acid, and cystatin, inhibited protease activity as well as in vitro excystation and infection in a cell culture assay. Several mechanisms may result in the successful inhibition of infection and excystation by hydrogen peroxide treatment, including: oxidation of oocyst wall proteins or lipids, chelating of cations necessary for infection, or hydroxyl radical–induced DNA damage to sporozoites, or both.
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August 2004
RESEARCH NOTES|
August 01 2004
Effect of Hydrogen Peroxide and Other Protease Inhibitors on Cryptosporidium parvum Excystation and In Vitro Development
K. E. Kniel;
K. E. Kniel
aDepartment of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061
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S. S. Sumner;
S. S. Sumner
aDepartment of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061
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M. D. Pierson;
M. D. Pierson
aDepartment of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061
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A. M. Zajac;
A. M. Zajac
aDepartment of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061
bVirginia–Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061
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C. R. Hackney;
C. R. Hackney
aDepartment of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061
cUnited States Department of Agriculture Environmental Microbial Safety Laboratory, Beltsville, Maryland 20705. kniel@udel.edu
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R. Fayer;
R. Fayer
aDepartment of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061
bVirginia–Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061
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D. S. Lindsay
D. S. Lindsay
aDepartment of Food Science and Technology, Virginia Tech, Blacksburg, Virginia 24061
bVirginia–Maryland Regional College of Veterinary Medicine, Virginia Tech, Blacksburg, Virginia 24061
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J Parasitol (2004) 90 (4): 885–888.
Citation
K. E. Kniel, S. S. Sumner, M. D. Pierson, A. M. Zajac, C. R. Hackney, R. Fayer, D. S. Lindsay; Effect of Hydrogen Peroxide and Other Protease Inhibitors on Cryptosporidium parvum Excystation and In Vitro Development. J Parasitol 1 August 2004; 90 (4): 885–888. doi: https://doi.org/10.1645/GE-203R1
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