Calreticulin is an endoplasmic reticulum protein involved in the homeostasis of intracellular Ca++ and other physiological processes. A complementary DNA clone containing the complete coding sequence of Taenia solium calreticulin (TsCRT) was isolated and characterized. Recombinant TsCRT was expressed in bacteria as a 50-kDa protein that specifically bound calcium when tested in a radioassay. The deduced amino acid sequence has 47–50% identity with other reported calreticulins. Poor recognition of TsCRT by human and pig sera with confirmed cysticercosis discourages its use for diagnosis of the disease. However, further characterization and localization studies could provide insights into the role of TsCRT in T. solium physiology and host–parasite interactions.
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August 2004
RESEARCH NOTES|
August 01 2004
Cloning, Characterization, and Functional Expression of Taenia Solium Calreticulin
Fela Mendlovic;
Fela Mendlovic
aDepartamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF 04510, México
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Pedro Ostoa-Saloma;
Pedro Ostoa-Saloma
bDepartamento de Inmunología, Instituto de Investigaciones Biomédicas, UNAM, México DF 04510, México
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Carlos F. Solís;
Carlos F. Solís
bDepartamento de Inmunología, Instituto de Investigaciones Biomédicas, UNAM, México DF 04510, México
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Joel Martínez-Ocaña;
Joel Martínez-Ocaña
cDirección de Investigación, Hospital General “Dr. Manuel Gea González”, SSA, México DF 14000, México
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Ana Flisser;
Ana Flisser
aDepartamento de Microbiología y Parasitología, Facultad de Medicina, UNAM, México DF 04510, México
cDirección de Investigación, Hospital General “Dr. Manuel Gea González”, SSA, México DF 14000, México
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Juan P. Laclette
Juan P. Laclette
bDepartamento de Inmunología, Instituto de Investigaciones Biomédicas, UNAM, México DF 04510, México
dTo whom correspondence should be addressed. laclette@servidor.unam.mx
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J Parasitol (2004) 90 (4): 891–893.
Citation
Fela Mendlovic, Pedro Ostoa-Saloma, Carlos F. Solís, Joel Martínez-Ocaña, Ana Flisser, Juan P. Laclette; Cloning, Characterization, and Functional Expression of Taenia Solium Calreticulin. J Parasitol 1 August 2004; 90 (4): 891–893. doi: https://doi.org/10.1645/GE-3325RN
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