Sarcocystis neurona is the primary parasite associated with equine protozoal myeloencephalitis (EPM). This is a commonly diagnosed neurological disorder in the Americas that infects the central nervous system of horses. Current serologic assays utilize culture-derived parasites as antigen. This method requires large numbers of parasites to be grown in culture, which is labor intensive and time consuming. Also, a culture-derived whole-parasite preparation contains conserved antigens that could cross-react with antibodies against other Sarcocystis species and members of Sarcocystidae such as Neospora spp., Hammondia spp., and Toxoplasma gondii. Therefore, there is a need to develop an improved method for the detection of S. neurona–specific antibodies. The sera of infected horses react strongly to surface antigen 1 (SnSAG1), an ∼29-kDa protein, in immunoblot analysis, suggesting that it is an immunodominant antigen. The SnSAG1 gene of S. neurona was cloned, and recombinant S. neurona SAG1 protein (rSnSAG1-Bac) was expressed with the use of a baculovirus system. By immunoblot analysis, the rSnSAG1-Bac antigen detected antibodies to S. neurona from naturally infected and experimentally inoculated equids, cats, rabbit, mice, and skunk. This is the first report of a baculovirus-expressed recombinant S. neurona antigen being used to detect anti–S. neurona antibodies in a variety of host species.
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October 2004
IMMUNOLOGY|
October 01 2004
ANTIGENIC EVALUATION OF A RECOMBINANT BACULOVIRUS-EXPRESSED SARCOCYSTIS NEURONA SAG1 ANTIGEN
G. D. Gupta;
G. D. Gupta
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211. marsh.2061@osu.edu
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J. Lakritz;
J. Lakritz
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211. marsh.2061@osu.edu
* Department of Large Animal Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210
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W. J. Saville;
W. J. Saville
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211. marsh.2061@osu.edu
† Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210
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R. S. Livingston;
R. S. Livingston
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211. marsh.2061@osu.edu
‡ Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211
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J. P. Dubey;
J. P. Dubey
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211. marsh.2061@osu.edu
§ Animal Parasitic Diseases Laboratory, Animal and Natural Resources Institute, Agricultural Research Service, United States Department of Agriculture, Beltsville, Maryland 20705
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J. R. Middleton;
J. R. Middleton
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211. marsh.2061@osu.edu
‖ Department of Veterinary Medicine and Surgery, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211
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A. E. Marsh
A. E. Marsh
Department of Veterinary Pathobiology, College of Veterinary Medicine, University of Missouri, Columbia, Missouri 65211. marsh.2061@osu.edu
† Department of Veterinary Preventive Medicine, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210
# To whom correspondence should be addressed
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J Parasitol (2004) 90 (5): 1027–1033.
Citation
G. D. Gupta, J. Lakritz, W. J. Saville, R. S. Livingston, J. P. Dubey, J. R. Middleton, A. E. Marsh; ANTIGENIC EVALUATION OF A RECOMBINANT BACULOVIRUS-EXPRESSED SARCOCYSTIS NEURONA SAG1 ANTIGEN. J Parasitol 1 October 2004; 90 (5): 1027–1033. doi: https://doi.org/10.1645/0022-3395(2004)090[1027:AEOARB]2.0.CO;2
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