Parasite Genomics Protocols, Volume 270, Methods In Molecular Biology, edited by S. E. Melville. Humana Press, Totowa, New Jersey. 2004. 452 p. $119.50. ISBN 1-58829-062-X.
This volume in the Methods in Molecular Biology series contains 24 chapters describing a wide array of protocols in the field of parasite genomics. The 24 chapters include a variety of genomics, functional genomics, and postgenomics protocols such as analysis of gene function using RNA interference, expressed sequence tags, RACE and RAGE cloning, microarray analyses, gene deletion and tagging, and chromosome fragmentation. In addition to the laboratory protocols, there are 3 reviews that describe the genome data that are currently available and focus largely on genome databases, web-based resources, and genome annotation. Each chapter begins with a brief summary and an introduction that describes the usefulness of the protocol, principles of the techniques, required equipment and reagents, and potential problems that might be encountered. When...