Theileria sergenti causes persistent theileriosis in cattle, characterized by fever and chronic anemia. Theileriosis causes losses in feed efficiency and growth retardation through cycling infections in endemic areas. Among several major proteins of T. sergenti merozoites, the surface protein p33 is reported to be the most immunogenic. In this study, we investigated the use of p33 as a diagnostic antigen in a latex agglutination test to monitor antibodies against T. sergenti. When compared with TaqMan polymerase chain reaction, the sensitivity and specificity of the latex agglutination test were 86.5 and 92.5%, respectively. An epidemiological survey using the latex agglutination test was conducted with 1,046 sera collected from 4 slaughterhouses and 2 individual pasture farms throughout South Korea; 27.3% of samples were seropositive, depending on the areas in which the cattle were raised. This study indicated that the latex agglutination test could be used as a convenient tool for epidemiological monitoring of T. sergenti infections in the field.
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February 2005
THERAPEUTICS-DIAGNOSTICS|
February 01 2005
SEROLOGICAL INVESTIGATION OF THEILERIA SERGENTI USING LATEX AGGLUTINATION TEST IN SOUTH KOREA
Wooseog Jeong;
Wooseog Jeong
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 427-824, South Korea. [email protected]
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Chang Hee Kweon;
Chang Hee Kweon
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 427-824, South Korea. [email protected]
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Jong Man Kim;
Jong Man Kim
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 427-824, South Korea. [email protected]
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Hwan Jang;
Hwan Jang
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 427-824, South Korea. [email protected]
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Sang Gi Paik
Sang Gi Paik
National Veterinary Research and Quarantine Service, Anyang, Gyeonggi 427-824, South Korea. [email protected]
* Department of Biology, College of Natural Sciences, Chungnam National University, Taejon 305-764, South Korea
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J Parasitol (2005) 91 (1): 164–169.
Citation
Wooseog Jeong, Chang Hee Kweon, Jong Man Kim, Hwan Jang, Sang Gi Paik; SEROLOGICAL INVESTIGATION OF THEILERIA SERGENTI USING LATEX AGGLUTINATION TEST IN SOUTH KOREA. J Parasitol 1 February 2005; 91 (1): 164–169. doi: https://doi.org/10.1645/GE-3294
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