Autofluorescence of Toxoplasma gondii and Neospora caninum was studied by fluorescence microscopy during their differentiation from tachyzoites to bradyzoites in vitro using Vero as host cells. Stage conversion into bradyzoites and cysts was confirmed by immunofluorescent microscopy and Western blot analysis using SAG1- and BAG1-specific antibody, respectively. From day 4 postinfection (PI), pale blue autofluorescence of the bradyzoites and tissue cysts was observed with UV light at 330– 385 nm, which coincided with the onset of cyst development. This autofluorescence under UV light of bradyzoites and tissue cysts increased in intensity from days 8 to 10 PI. In contrast to the autofluorescence shown by bradyzoites and cysts, tachyzoites and parasitophorous vacuoles containing tachyzoites never autofluoresced at any time examined. Autofluorescence of the cystic stages was of sufficient intensity and duration to allow the detection of cysts and bradyzoites of T. gondii and N. caninum. In this study, we describe for the first time the autofluorescence properties of in vitro–induced bradyzoites and cysts of T. gondii and N. caninum.
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February 2005
DEVELOPMENT|
February 01 2005
AUTOFLUORESCENCE OF TOXOPLASMA GONDII AND NEOSPORA CANINUM CYSTS IN VITRO
Ying Lei;
Ying Lei
Department of Cell and Molecular Biology, University of Technology, Sydney, Gore Hill, New South Wales 2065, Australia. [email protected]
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Mary Davey;
Mary Davey
Department of Cell and Molecular Biology, University of Technology, Sydney, Gore Hill, New South Wales 2065, Australia. [email protected]
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John Ellis
John Ellis
Department of Cell and Molecular Biology, University of Technology, Sydney, Gore Hill, New South Wales 2065, Australia. [email protected]
* To whom correspondence should be addressed. Department of Cell and Molecular Biology, Faculty of Science, University of Technology, Sydney, NSW 2065, Australia
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J Parasitol (2005) 91 (1): 17–23.
Citation
Ying Lei, Mary Davey, John Ellis; AUTOFLUORESCENCE OF TOXOPLASMA GONDII AND NEOSPORA CANINUM CYSTS IN VITRO. J Parasitol 1 February 2005; 91 (1): 17–23. doi: https://doi.org/10.1645/GE-355R
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