Cysts of Giardia duodenalis were collected in Mexico from symptomatic children (n = 9) and from pet dogs (n = 5), and they were directly characterized by nested polymerase chain reaction (PCR) amplification of the β-giardin gene. Eight isolates of human origin established as in vitro cultures and 2 reference strains, representing assemblages A and B of G. duodenalis, were also analyzed. PCR–restriction fragment length polymorphism showed that all isolates belonged to assemblage A. Sequence analyses indicated that the large majority of isolates were of the A1 genotype; interestingly, 2 human isolates displayed the A3 genotype, which has been previously identified in human isolates from Italy. The presence of cysts of the A1 and A3 genotypes in isolates from pet dogs is consistent with their role as reservoirs for human infection, although further studies are needed to confirm the occurrence of zoonotic transmission. Remarkably, cysts of assemblage B have not been found in any of the Mexican isolates studied to date.
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February 2005
Research Article|
February 01 2005
Genotyping of Giardia duodenalis From Humans and Dogs From Mexico Using a β-Giardin Nested Polymerase Chain Reaction Assay
Marco Lalle
;
Marco Lalle
aDepartment of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy
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Enedina Jimenez-Cardosa
;
Enedina Jimenez-Cardosa
bLaboratorio de Investigación en Parasitología, Hospital Infantil de México “Federico Gómez,” México, 06720 D.F. México
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Simone M. Cacciò
;
Simone M. Cacciò
aDepartment of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanità, viale Regina Elena 299, 00161 Rome, Italy
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Edoardo Pozio
Edoardo Pozio
cTo whom correspondence should be addressed. pozio@iss.it
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J Parasitol (2005) 91 (1): 203–205.
Citation
Marco Lalle, Enedina Jimenez-Cardosa, Simone M. Cacciò, Edoardo Pozio; Genotyping of Giardia duodenalis From Humans and Dogs From Mexico Using a β-Giardin Nested Polymerase Chain Reaction Assay. J Parasitol 1 February 2005; 91 (1): 203–205. doi: https://doi.org/10.1645/GE-293R
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