A gene encoding a copper/zinc superoxide dismutase (Cu/ Zn-SOD) of a filarial nematode, Brugia malayi, has been isolated and the biochemical properties of a functionally expressed recombinant enzyme were investigated. The cloned complementary DNA contained a single open reading frame of 477 bp encoding 158 amino acids (aa), which conserved metal-binding residues as well as residues specific for Cu/Zn-SODs. Comparison of the deduced aa sequence of the enzyme with that of other helminthes species, including filarial worms, exhibited high degree of similarities (49–98%). Recombinant enzyme of 32 kDa had an isoelectric point of 6.6 and was shown to consist of 2 subunits linked by interchain disulfide bonds. Enzyme activity of the recombinant protein was inhibited by potassium cyanide and hydrogen peroxide but not by sodium azide. It showed a wide range of pH optima, i.e., 7.0–11.0 and was highly resistant to heat inactivation.
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February 2005
RESEARCH NOTES|
February 01 2005
Functional Expression of a Recombinant Copper/Zinc Superoxide Dismutase of Filarial Nematode, Brugia malayi
W-G. Lee;
W-G. Lee
aDepartment of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
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J-H. Hwang;
J-H. Hwang
bLaboratory of Animal Resources, National Institute of Toxicological Research, Korea Food and Drug Administration, Seoul 122-704, Korea
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B-K. Na;
B-K. Na
cDepartment of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon 440-746, Korea
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J-H. Cho;
J-H. Cho
aDepartment of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
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H-W. Lee;
H-W. Lee
aDepartment of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
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S-H. Cho;
S-H. Cho
aDepartment of Tropical and Endemic Parasitic Diseases, National Institute of Health, Seoul 122-701, Korea
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Y. Kong;
Y. Kong
cDepartment of Molecular Parasitology and Center for Molecular Medicine, Sungkyunkwan University School of Medicine and Samsung Biomedical Research Institute, Suwon 440-746, Korea
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C-Y. Song;
C-Y. Song
dDepartment of Biology, College of Natural Science, Chung-Ang University, Seoul 156-756, Korea
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T-S. Kim
T-S. Kim
eTo whom correspondence should be addressed. [email protected]
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J Parasitol (2005) 91 (1): 205–208.
Citation
W-G. Lee, J-H. Hwang, B-K. Na, J-H. Cho, H-W. Lee, S-H. Cho, Y. Kong, C-Y. Song, T-S. Kim; Functional Expression of a Recombinant Copper/Zinc Superoxide Dismutase of Filarial Nematode, Brugia malayi. J Parasitol 1 February 2005; 91 (1): 205–208. doi: https://doi.org/10.1645/GE-285R
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