Liposome-associated ganglioside antigens (ganglioside GM1 or bovine brain gangliosides) were prepared to facilitate the potential protective efficacy for Trypanosoma brucei. Mice were immunized with liposome-associated ganglioside GM1 or bovine brain gangliosides intraperitoneally (i.p.). After immunization, significantly higher antigen-specific IgG and IgM antibodies were detected in sera than in the nonimmunized control group. When sera from immunized mice were analyzed for isotype distribution, antigen-specific IgG1, IgG2a, and IgG3 antibody responses were also noted. After immunization, mice were challenged i.p. with 1 × 102 cells of T. brucei. Sixty percentage of liposome-associated ganglioside GM1–immunized mice survived the infection, and all the mice immunized with bovine brain gangliosides–containing liposomes survived. However, all control mice died within 7 days after infection. These data demonstrate that liposomes containing ganglioside antigens have the potential usefulness for the induction of a protective immune response against T. brucei infection and suggest the possibility of developing vaccines that may ultimately be used for the prevention of trypanosomiasis.
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February 2005
Research Article|
February 01 2005
PROTECTIVE EFFECT OF ANTIGANGLIOSIDE ANTIBODIES AGAINST EXPERIMENTAL TRYPANOSOMA BRUCEI INFECTION IN MICE
Akiko Uemura;
Akiko Uemura
Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
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Shinobu Watarai;
Shinobu Watarai
Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
* To whom correspondence should be addressed
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Yoshihiro Ohnishi;
Yoshihiro Ohnishi
Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
† Laboratory of International of Epidemics, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan
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Hiroshi Kodama
Hiroshi Kodama
Laboratory of Veterinary Immunology, Division of Veterinary Science, Graduate School of Agriculture and Biological Sciences, Osaka Prefecture University, Sakai, Osaka 599-8531, Japan. swatarai@vet.osakafu-u.ac.jp
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J Parasitol (2005) 91 (1): 73–78.
Citation
Akiko Uemura, Shinobu Watarai, Yoshihiro Ohnishi, Hiroshi Kodama; PROTECTIVE EFFECT OF ANTIGANGLIOSIDE ANTIBODIES AGAINST EXPERIMENTAL TRYPANOSOMA BRUCEI INFECTION IN MICE. J Parasitol 1 February 2005; 91 (1): 73–78. doi: https://doi.org/10.1645/GE-3375
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