Sequence analysis of the ribosomal DNA second internal transcribed spacer (ITS 2) region in 2 spatially distinct populations of Amblyomma americanum (L.) revealed intraspecific variation. Nucleotide sequences from multiple DNA extractions and several polymerase chain reaction amplifications of eggs from mixed-parentage samples from both populations of ticks revealed that 12 of 1,145 (1.0%) sites varied. Three of the 12 sites of variation were distinct between the 2 A. americanum populations, which corresponded to a rate of 0.26%. Phylogenetic analysis based on ITS 2 sequences provided strong support (i.e., bootstrap value of 80%) that wild A. americanum clustered into a distinguishable group separate from those derived from colony ticks.
SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE)
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M. V. Reichard, A. A. Kocan, R. A. Van Den Bussche, R. W. Barker, J. H. Wyckoff, S. A. Ewing; SEQUENCE VARIATION OF THE RIBOSOMAL DNA SECOND INTERNAL TRANSCRIBED SPACER REGION IN TWO SPATIALLY DISTINCT POPULATIONS OF AMBLYOMMA AMERICANUM (L.) (ACARI: IXODIDAE). J Parasitol 1 April 2005; 91 (2): 260–263. doi: https://doi.org/10.1645/GE-292R1
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